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Abstracts / Sleep Medicine 40 (2017) e186ee363
countries in comparison to Caucasian population, suggesting different racial factors in RLS.18-24 Three studies from Asian population (Korean and Taiwan of China) confirmed the association of BTBD9, MEIS1 and PTPRD with RLS in Asian population.17,25,26 However, two studies from Taiwan of China only focused on certain types of RLS patients, for example, RLS with migraine or renal dysfunction associated RLS. It remains unknown whether the genetic risk factors of all primary RLS are the same as certain types of RLS. Thus, in this study, we selected 20 SNPs within the above six suspected RLS risk genetic loci (MEIS1, BTBD9, PTPRD, MAP2K5/SKOR1, TOX3 and Intergenic region of 2p14) to further test the relationship of these genetic risk factors with primary RLS in Chinese population. Materials and methods: A total of 116 RLS patients and 200 controls were recruited and the diagnosis of RLS was based on the criteria of International RLS Study Group. Polymer chain reaction (PCR) and sequencing were used to detect 19 single nucleotide polymorphisms (SNPs) in six genetic loci (MEIS1, BTBD9, PTPRD,MAP2K5/SKOR1, TOX3, and Intergenic region of 2p14). Results: Our study found that one SNP increased the risk of RLS in Chinese population: rs6494696 of MAP2K5/SKOR1 (odds ratio [OR] ¼ 0.09, p < .0001, recessive model). A further meta-analysis of RLS in Asian population found that two SNPs of BTBD9 increased the risk of RLS: rs9296249 of BTBD9 (OR ¼ 1.44, p ¼ .000, T allele), rs9357271 of BTBD9 (OR ¼ 1.38, p ¼ .021, dominant model). Conclusions: Our results suggested that BTBD9 and MAP2K5/SKOR1 are associated with RLS in Asian population. Unfortunately, we did not replicate the association of MEIS1, PTPRD, TOX3/BC034767 and intergenic region of 2p14 with RLS. Heterogeneity of ethnic origin and small sample size might account for the differences of our results with others. More large RLS cohorts are needed to explore the genetic risk factors for RLS in Asian population in the future. Acknowledgements: We would like to thank all subjects participating in this study and Dr. Yue Huang from UNSW Australia for proof reading. GL and HT collected the RLS and control data, performed the statistical analysis and drafted the manuscript. CW, XQ and JC collected the RLS data. SC supervised the study and revised the manuscript. JM designed the study, double-checked the statistical analysis and revised the manuscript. Insomnia ANALYST RELATIONSHIP BETWEEN FIVE-PATTERN PERSONALITY AND PITTSBURGH SLEEP QUALITY INDEX (PSQI) OF INSOMNIA PATIENTS G.-X. Li 1, Y.-L. Liu 2, W.-D. Wang 1, J. Wang 1, Y. Birling 2, C.-Y. Wang 1, W.-L. Qin 2. 1 Psychology and Sleep Department, China; 2 China Academy of Chinese Medical Sciences, Guang'an Men Hospital, Beijing, China Introduction: Analyst relationship between Five-Pattern Personality and Pittsburgh Sleep Quality Index(PSQI) of insomnia patients. Materials and methods: Recruited totally 240 insomnia patients. The Five-Pattern Personality scales and (PSQI) were used to evaluate the FivePattern Personality and observe the sleep index. The correlation was analyzed. Results: Scores of Taiyin dimension was positively correlated with sleep disturbance and daytime function (P< 0.05). Scores of Shaoyang, Shaoyin, YinYang balance dimension negative correlation with daytime function (P< 0.05). YinYang balance dimension was negative correlation with PSQI total score. Conclusion: Five-Pattern Personalities are correlated with PSQI. The result preliminary hint that each kind of personalities can be insomnia. However, Taiyin personality are more likely to suffer from insomnia. Acknowledgements: Research was funded by the following project: Psychology and Sleep Department of Guang'an men Hospital China Academy of Chinese Medical Sciences-Insomnia Clinic Research Base on the "Courage Determining Judgment and Response” (Grant No. 2016S358);Doctor Creating Product-Research on Theory and Clinic of Gallbladder Governs Decision TCM Psychology(Grant No. CX201403).And thanks to all the members cooperation and the participants who help us accomplish the study.
Basic Research TRADITIONAL CHINESE MEDICINE “COURAGE JUDGMENT AND RESPONSE” AND INSOMNIA
DETERMINING
G.-X. Li, W.-D. Wang, J. Wang, X.-Y. Lv, F. Feng, C.-Y. Wang, Y.-L. Liu, W.-L. Qin. Guang'anmen Hospital China Academy of Chinese Medical Sciences, Beijing, China Introduction: Before 2000 “The Yellow Emperor's Classic of Internal Medicine” recorded “Courage Determining Judgment and Response”, and in TCM theory, if this function is abnormal, it will cause disease. According to this, we can speculate that there is also a close relationship between insomnia and “Courage Determining Judgment and Response". Materials and methods: On the basis of previous studies (Including China and the international), our team explored the relationship between “Courage Determining Judgment and Response”. Results: Timidity can cause judgment aberration, and the aberration can make a miscalculate the threat. They will judge security situation as dangerous. In addition to sleep fear and anxiety, The patient will also take the sleep-related wrong behavioral response. The negative emotions make patients to be excited when they should be asleep, and are more sensitive. Improper behavior can weaken the conditioned stimulus of sleep. Not only that, the insomnia experience as a negative thing, each day is aggravating the patient's fear. The patient's decision disorder increases the uncertainty. Patients are more unable to determine the objectivity of their insomnia related judgment. Therefore, timidity is a key link in patients' insomnia, judgment aberration is the direct factor. The main performance of judgment disorder which is caused by timidity is the subjective and objective does not conform to. This produce a series of negative emotion and improper behavior, and cause insomnia, continuous and aggravating, and become complicated and difficult to cure. Conclusions: There is a close relationship between insomnia and “Courage Determining Judgment and Response”. In spite of this, further research remains to be done. Acknowledgements: Research was funded by the following project: Psychology and Sleep Department of Guang'an men Hospital China Academy of Chinese Medical Sciences-Insomnia Clinic Research Base on the “Courage Determining Judgment and Response” (Grant No. 2016S358); Doctor Creating Product-Research on Theory and Clinic of Gallbladder Governs Decision TCM Psychology (Grant No. CX201403). Sleep Breathing Disorders EFFECTS OF HABENULAR STIMULATION FREQUENCIES ON OBSTRUCTIVE SLEEP APNEA INDUCED BY STIMULATION OF INSULAR CORTEX IN RAT M. Li, K. Jing. Respirology, The First Affiliated Hospital of Jilin University, Changchun, China Introduction: Our previous study has shown that obstructive sleep apnea (OSA) events could be induced following stimulation of cerebral insular cortex (Hc) in rat .The current study is to investigate the effects of different stimulation frequencies of the habenular (Hb) on OSA induced by stimulation of Hc Materials and methods: After OSA was induced by stimulating the insular cortex (Ic) with concentric stimulating electrodes at 100 Hz in rats, the Hb was stimulated at different frequencies (50 Hz, 120 Hz, 130 Hz and 280 Hz). The changes of apnea events and electromyography (EMG) of the genioglossus were compared before and after stimulation of the Hb. Results: With stimulation of the Ic at 100 Hz apnea events was successfully induced with disappearance of EMG of the genioglossus. After stimulation of the Hb at 130 Hz, apnea events disappeared with significantly increased gengioglossal EMG. However, such a change failed to be found at the stimulation frequencies of 50 Hz, 120 Hz, and 280 Hz. Conclusions: Stimulation of Hb at the frequency of 130 Hz could effectively inhibit OSA events induced by stimulation of Ic in rat. Acknowledgements: The authors are grateful for the experimental assistance from Department of Physiology of Jilin University, China.
