2274
COMMUNICATIONS TO THE
Vol. 71
EDITOR
c, 67.59; H,6.93; C1,
11.08; N, 4.38. Found: (normal form) C, 67.55; H, 6.94; C1, 11.44; N, 4.27; (isoform) C, 67.50; H, 6.93; C1, 11.07; N, 4.31.
However, the bovine B substances cross reacted extensively but not completely with anti-horse B and showed a much higher capacity to precipitate anti-B per unit weight than would have been expected from the hemagglutination-inhibition test. DEPARTMENT OF CHEMISTRY UNIVERSITY OF MICHIGAN W. E. BACHMANN The table shows the anti-horse B nitrogen preANNARBOR,MICHIGAN FAUSTORAMIREZ cipitable from 2.0 ml. serum by various quantities RECEIVED APRIL25, 1949 of bovine B, as compared with horse B. The cow B precipitates anti-B as evidenced in the supernatant by the reduction in anti-B agglutinins and A MATERIAL IN BOVINE STOMACHS RELATED TO in antibody N precipitable by homologous horse BLOOD GROUP B SUBSTANCE' B substance. Excess bovine B inhibits precipitaSir : tion and reduces the quantity of antibody preBlood group A substances in cattle have been cipitable from the supernatant by horse B. studied2s8and the possible presence of blood group DEPARTMENTS OF BACTERIOLOGY AND NEUROLOGY B substance noted.4 Our findings using hemag- COLLEGE OF PHYSICIANS AND SURGEONS UNIVERSITY, SAMM. BEISER~ glutination-inhibition indicate that substances COLUMBIA AND THE NEUROLOGICAL ELVINA. KABAT with either blood group A, B, 0, AO, BO, or with PRESBYTERIAN HOSPITAL, NEWYORK negligible activity can be obtained by an identical INSTITUTE, RECEIVED APRIL5, 1949 procedureKfrom different individual bovine stom(9) American Academy of Allergy Fellow, 1948. 1949. achs (abomasus). The purifieds*'substances were analyzed: N, 5.0-7.270; reducing sugar as glucose (after hydrolysis) 51-60% ; hexosamine ACTIVITY OF 1,1,4,+TETRA-(P(after hydrolysis) 23-34% ; methylpentose 1.5- INSECTICIDAL CHLOROPHENYL)-2,2,3,3-TETR.ACHLOROBUTANE 5.2%. Analytical data of hog6 and human' substances are similar, except that their methyl- Sir : The recent communication by Fleck' prompts pentose contents are higher.s Four of nine preparations showed only blood me to report that 1,1,4,4,tetra-(p-~hlorophenyl)group B activity, which was 1 4 % the activity of 2,2,3,3-tetrachlorobutane(11), closely related to B substances from human saliva or horse stomach the compound 111 which is shown by the above as determined by hemagglutination-inhibition. author to be produced' in the ultraviolet irradiation of D D T (I), has been found to possess deTABLEI cided insecticidal activity. ANTIBODYN PRECIPITATED FROM 2.0 ML. SERUM OF A HUMANOF BLOODGROUPA IMMUNIZED WITH HORSEB
SUBSTANCE
Bovine substance added, pg
25 50 100 250 500
VOl. of supernatant giving Antibody N detectable precipitable Antibody agglutination of from supernatant by N precipihuman B tated, pg cells,' ml. horse B, pg
Total antibody N, pg
0.05 .1 .2 .2 .2
35.6 33.9 25.i 21.2 12.5
24.6 29.0 24.5 19.6 10.4
11.0 4.9 1.2 1.6 2.1
Horse B 60 32.5* 0.003 ml. of original serum is capable of agglutinating Point of maximum prethe quantity of B cells used. cipitation. No agglutinins for human B cells are detectable in the supernatant. 0
(1) Aided by grants from the United States Public Health Service and the William J. Matheson Commission. (2) G. Hartmann, D d . Kgl. Vidcnskab. Sclskab B i d . M c d d . , Copenhagen, 16, No. 10 (1941). (3) E.Jorpes and T. Thaning, J . Immunol., 61, 215, 221 (1945). (4) K. Landsteiner and M. W. Chase, J . Ex?. Mcd., 6% 813 (1936). (5) W.T. J. Morgan and H. K. King, Biochcm. J . , 87, 640 (1943). (6) A. Bendich. E. A. Kabat and A. E. Bezer, J . E x p . Mcd., 83, 485 (1946). (7) E. A. Kabat, A. Bendich, A. E. Bezer end S. M. Beiser, ibid., 86, 685 (1947). (8) H. Baer, 2.Dische and E. A. Kabat, ibid., 88, 59 (1948).
Rd2HCCla (1) RzCHCClzCCliCHCRa (11) R2CHCCl=CClCHR2 (111)
R
P-ClCaH4-
Although I have been aware of this fact for a year, only preliminary tests are a t hand, because much time has been consumed in various attempts to obtain a more satisfactory procedure for the preparation of I1 than the method of Brand and Bausch.2a However, recently, a test was performed by Prof. E. Delvaux (Agronomic Institute, Louvain) which gave evidence that I1 is as toxic as D D T to Drosophila melanogaster Meig., though slightly weaker in knock-down activity. The insecticidal power of I1 suggests that it may contribute possibly to some extent to the well-known high residual effect of DDT, because it is not objectionable a t all to assume that I1 is an intermediate product in the formation of 111,2b which is provisionally considered as being much less effective than 11, by analogy with the strong decrease in activity which accompanies the conversion of D D T into the corresponding ethylenic derivative. Besides, in connection with the problem of the relation between insecticidal activity and chemical constitution an extensive review of the litera(1) Fleck, THISJOURNAL, 71, 1034 (1949). (2) (a) Brand and Bausch, J . prakf. Ckcm., 137, 232 (1930): (b) 127, 233 (1930).
