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Bioactive Constituents, Metabolites, and Functions
A novel ex vivo experimental setup to investigate how food components stimulate the enteroendocrine secretions of different intestinal segments Iris Ginés, Katherine Gil-Cardoso, Paula Robles, Luis Arola, Ximena Terra, Mayte Blay, Anna Ardévol, and Montserrat Pinent J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b03046 • Publication Date (Web): 27 Aug 2018 Downloaded from http://pubs.acs.org on August 29, 2018
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Journal of Agricultural and Food Chemistry
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A novel ex vivo experimental setup to assay the vectorial transepithelial
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enteroendocrine secretions of different intestinal segments
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Ginés, Iris;1 Gil-Cardoso, Katherine;1 Robles, Paula;1 Arola, Luis;2 Terra, Ximena;1
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Blay, Mayte;1 Ardévol, Anna;1 Pinent Montserrat1
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Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Tarragona (Spain).
MoBioFood Research Group.
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Nutrigenomics Research Group. Departament de
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Corresponding author: Anna Ardévol, Departament de Bioquímica i Biotecnologia,
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Universitat Rovira i Virgili, c/ Marcellí Domingo, nº1, 43007, Tarragona, Spain. Phone:
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34 977 55 9566, Email:
[email protected] 12 13
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Abstract
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The enteroendocrine system coordinates gastrointestinal (GI) tract functionality and the whole
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organism. However, the scarcity of enteroendocrine cells and their scattered distribution make
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them difficult to study. Here, we glued segments of the GI wall of pigs to a silicon tube,
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keeping the apical and the basolateral sides separate. The fact that there was less than 1% of
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70-kDa fluorescein isothiocyanate (FITC)-dextran on the basolateral side proved that the gluing
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was efficient. Since the lactate dehydrogenase leakage at basolateral side was lower than 0.1%
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(1.40 ± 0.17 nKatals)it proved that the tissue was viable. The intestinal barrier function was
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maintained as it is in segments mounted in Ussing chambers (the amount of Lucifer Yellow
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crossing it, was similar between them; respectively % LY: 0.48 ±0.13; 0.52 ± 0.09; p> 0.05).
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Finally, apical treatments with two different extract produced differential basolateral
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enterohormone secretions (basolateral PYY secretion vs control; animal extract: 0.35 ± 0.16;
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plant extract: 2.5 ± 0.74; p
