Biochemistry 1996, 35, 14259-14267
14259
A One-Site, Two-State Model for the Binding of Anions in Photosystem II† Katrin Lindberg and Lars-Erik Andre´asson* Department of Biochemistry and Biophysics, Go¨ teborg UniVersity and Chalmers UniVersity of Technology, Medicinaregatan 9C, S-413 90 Go¨ teborg, Sweden ReceiVed May 27, 1996; ReVised Manuscript ReceiVed August 26, 1996X
Photosystem II membranes, dialyzed against a Cl--free buffer to remove bound Cl-, lost about 65% of the control activity. A light-intensity study of the Cl--free membranes showed that all PS II centers were able to evolve oxygen at about 35% of the control rate when measured in Cl--free medium. The Cl--depleted membranes were immediately (
