Letter pubs.acs.org/NanoLett
A Protein Rotaxane Controls the Translocation of Proteins Across a ClyA Nanopore Annemie Biesemans,‡,§ Misha Soskine,†,§ and Giovanni Maglia*,†,‡ †
Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen, 9747 AG, Groningen, The Netherlands Department of Chemistry, University of Leuven, Leuven 3001, Belgium
‡
S Supporting Information *
ABSTRACT: Rotaxanes, pseudorotaxanes, and catenanes are supramolecular complexes with potential use in nanomachinery, molecular computing, and single-molecule studies. Here we constructed a protein rotaxane in which a polypeptide thread is encircled by a Cytolysin A (ClyA) nanopore and capped by two protein stoppers. The rotaxane could be switched between two states. At low negative applied potentials (
