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A Selective and Regenerable Surface Based on #Cyclodextrin for Low-Density Lipoprotein Adsorption Fei Fang, Xiao-Jun Huang, Yi Zong Guo, Xiao Hong, Hui Min Wu, Rong Liu, and Da Jing Chen Langmuir, Just Accepted Manuscript • DOI: 10.1021/acs.langmuir.8b00883 • Publication Date (Web): 20 Jun 2018 Downloaded from http://pubs.acs.org on July 4, 2018
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[Prepared as a full article for publication in Langmuir]a
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A Selective and Regenerable Surface Based on β-Cyclodextrin for
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Low-Density Lipoprotein Adsorption
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Fei Fang1, Xiao-Jun Huang1*, Yi-Zong Guo1, Xiao Hong 1, Hui-Min Wu1, Rong Liu2**, DaJing Chen3***,
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1, MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of
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Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
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2. Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom,
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Kowloon, Hong Kong.
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3, Medical School, Hangzhou Normal University, Hangzhou 311121, China
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*Corresponding
author at: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China E-mail address:
[email protected]. (X.J. Huang) **Corresponding author at: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong E-mail address:
[email protected] (R.Liu) ***Corresponding author at: Medical School, Hangzhou Normal University, Hangzhou 311121, China E-mail address:
[email protected] (D.J. Chen) 1
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Fig. 1. (A) UV–Vis spectra and (B) the linked fitting curve for LDL (c = 85 mg/L) in β-CD
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solutions with varying concentrations (bottom to top): 0, 0.5, 1, 3, 5, 7, 8, 10, 13, 17, and 18
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g/L and the kinetic analysis of the (C) LDL, (D) β-CD, (E) LDL-β-CD, and (F) the
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corresponding size distribution by intensity for LDL-β-CD
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Fig. 2. Circular dichroism spectra of LDL in different solutions: (top to bottom) LDL in PBS
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buffer solution (pH 7.4); LDL in 2.1 g/L β-CD/PBS; and LDL in 3.3 g/L β-CD/PBS.
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Fig. 3. Schematic illustration of surface modification of SH-CD via SAM procedures.
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(B)
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Fig. 4. Morphologies of the bare gold (A) and SH-CD (B) surface.
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Fig. 5. Typical example of SPR curve of LDL adsorption/desorption on SH-CD (A) and bare
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gold (B) surface
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Fig. 6.SPR equilibrium adsorption for LDL at 20μg/mL on β-CD-modified surface
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Fig. 7. (A)SPR curve of LDL adsorption on β-CD-modified surface with Phenylalanine (Phe)
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treatment, (B)detail information for Phe adsorption on cyclodextrin modified surface linked to
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figure (A).
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Fig. 8. SPR equilibrium adsorption for HDL at 20μg/mL on β-CD-modified surface
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Table 1 Secondary structures of LDL calculated based on circular dichroism spectra by using the
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CDSSTR program with reference set SDP48 (IBasis = 7). Sample
H(r)%
H(d)%
S(r)%
S(d)%
Trn%
Unrd%
NRMSD*
LDL
20.9
18.1
15.8
16.5
13.1
15.6
0.076
LDL-CD2.1
22.4
20.2
16.3
17.1
11.2
12.9
0.093
LDL-CD3.3
24.2
21.1
16.2
17.4
9.2
10.8
0.081
LDL-CD4.5
24.5
22.9
16.5
17.6
8.9
9.6
0.093
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H(r) = Regular α-Helix; H(d) = Distorted α-Helix;
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S(r) = Regular β-Strand; S(d) = Distorted β-Strand;
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Trn = Turns; Unrd = Unordered.
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*The NRMSD is a fit parameter which is a measure of the difference between the experimental
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ellipticities and the ellipticities of the back-calculated spectra for the derived structure.
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