Article pubs.acs.org/biochemistry
A Simple Method for Correction of Circular Dichroism Spectra Obtained from Membrane-Containing Samples Hirak Chakraborty and Barry R. Lentz* Department of Biochemistry and Biophysics and Program in Molecular and Cellular Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599-7260, United States ABSTRACT: Circular dichroism (CD) spectroscopy is an important technique in structural biology for examining folding and conformational changes of proteins in solution. However, the use of CD spectroscopy in a membrane medium (and also in a nonhomogeneous medium) is limited by (i) high light scattering and (ii) differential scattering of incident left and right circularly polarized light, especially at shorter wavelengths (
