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A simple strategy for rapid and sensitive detection of avian influenza A H7N9 virus based on intensitymodulated SPR biosensor and new generated antibody Ying-Feng Chang, Wen-Hung Wang, Yi-Wei Hong, Ruei-Yu Yuan, Kuan-Hsuan Chen, Yu-Wen Huang, Po-Liang Lu, Yen-Hsu Chen, Yi-Ming Arthur Chen, Li-Chen Su, and Sheng-Fan Wang Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.7b03934 • Publication Date (Web): 12 Jan 2018 Downloaded from http://pubs.acs.org on January 13, 2018
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Analytical Chemistry
A simple strategy for rapid and sensitive detection of avian influenza A H7N9 virus based on intensity-modulated SPR biosensor and new generated antibody Ying-Feng Chang1, #, Wen-Hung Wang2,3 #, Yi-Wei Hong3,4, Ruei-Yu Yuan3,4, Kuan-Hsuan Chen3, Yu-Wen *
Huang5, Po-Liang Lu2, 6, Yen-Hsu Chen2, 7, Yi-Ming Arthur Chen3, 7, Li-Chen Su8, , Sheng-Fan Wang 3, 4, 9
1
*
Bio-Analytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
2
Division of Infection Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan.
3
Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
4
Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
5
Dermatology department, Yuan’s general hospital, Kaohsiung 80249, Taiwan.
6
Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan.
7
Department of Microbiology and Immunology, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
8
Department of Optoelectric Physics, Chinese Culture University, Taipei 11114, Taiwan.
9
Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 81267, Taiwan.
#
These authors equally contributed to this work.
*Correspondence: Dr. Li-Chen Su (e-mail:
[email protected]), Department of Optoelectric Physics, Chinese Culture University, Taipei 11114, Taiwan. Tel: +886-2-28610511#25232; Fax: +886-2-2861-0577. Dr. Sheng-Fan Wang (e-mail:
[email protected]), Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. Tel: +886-7-3121101#2558; Fax: +886-7-322-2783.
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Abstract In 2013 a new reassortant avian influenza A H7N9 virus
emerged in China, causing human infection
with high mortality. An accurate and timely diagnosis is crucial for controlling the outbreaks of the disease. We therefore propose a simple strategy for rapidly and sensitively detecting the H7N9 virus using an intensity-modulated surface plasmon resonance (IM-SPR) biosensor integrated with a new generated monoclonal antibody. The novel antibody exhibits significant specificity to recognize H7N9 virus compared with other clinical human influenza isolates (p
