6974
Biochemistry 1993, 32, 6974-6981
Acrylamide Quenching of the Intrinsic Fluorescence of Tryptophan Residues Genetically Engineered into the Soluble Colicin E 1 Channel Peptide. Structural Characterization of the Insertion-Competent State7 A. R. Merrill,*J L. R. Palmer,$ and A. G. Szabos Guelph- Waterloo Centre for Graduate Work in Chemistry, Department of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario, Canada NI G 2W1,and Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada KIA OR6 Received February 10, 1993; Revised Manuscript Received April 16, I993
in vitro by acidic (
