Active Site Interactions Impact Phosphoryl Transfer during Replication

Oct 20, 2017 - Replicative DNA polymerases are able to discriminate between very similar substrates with high accuracy. One mechanism by which Escherichia coli DNA polymerase I checks for Watson-Crick geometry is through a hydrogen bonding fork betwe

Department of Biochemistry and Molecular Biology, Milton S. Hershey Medical Center, Pennsylvania. State University College of Medicine, Hershey, ...

Oct 20, 2017 - substrates, (2) the position of the nascent base pair, where the ..... During the pulse-chase experiments, 32P- labeled oligodeoxynucleotide was separated from unreacted [α-32P]. dCTP by PAGE. The relative extent of reaction was ... p

Sep 14, 2010 - Active Site Threonine Facilitates Proton Transfer during Dioxygen Activation at the ... showing active sites, Michaelis−Menten kinetics, time-dependent Mössbauer and ..... DuPont to pay $3.1 million penalty for Texas accident.

Sep 14, 2010 - Martinis , S. A., Atkins , W. M., Stayton , P. S., and Sligar , S. G. J. Am. Chem. ... Martinis, Susan A.; Atkins, William M.; Stayton, Patrick S.; Sligar, ...

Sep 14, 2010 - ... Medical Sciences and by a grant from Research Corporation (to M.H.S). ...... Marco Chino , Linda Leone , Ornella Maglio , Daniele D'Alonzo ...

May 8, 2012 - Judith P. Klinman , Adam R. Offenbacher , and Shenshen Hu ... H. Christine Lo , Jessica D. Ryan , John B. Kerr , Douglas S. Clark , Richard H.

(10-13) Alcohol dehydrogenases, which catalyze the stereospecific transfer of a hydride ... systems that have been perturbed from their optimal structure and dynamics. ... (39) Whereas the HLADH data were collected only at 25 °C, the use of .... the

May 8, 2012 - A growing body of data suggests that protein motion plays an important role in enzyme catalysis. Two highly conserved hydrophobic active site residues in the cofactor-binding pocket of ht-ADH (Leu176 and V260) have been mutated to a ser

May 8, 2012 - ... University of California, Berkeley, California 94720, United States .... Biochimica et Biophysica Acta (BBA) - Bioenergetics 2014 1837, 1730- ...