YOUNT
e t al.
Adenylyl Imidodiphosphate, an Adenosine Triphosphate Analog Containing a P-N-P Linkage* Ralph G. Yount,t Donner Babcock, Wm. Ballantyne, and Deanna Ojala
ABSTRACT: An analog of adenosine triphosphate, adenylyl imidodiphosphate (AMP-PNP), in which a "-grouping replaces the terminal bridge oxygen of the triphosphate chain has been synthesized by the reaction of imidodiphosphate with PI-adenosine P2-diphenyl pyrophosphate (Michelson, A. (1964), Biochim. Biophys. Acta 9/, 1). AMP-PNP is stable hydrolysis) for months at -20" as the tetrasodium salt and in solution a t 25" a t neutral pH for at least 16 hr. The principal nonenzymic breakdown products are inorganic phosphate and adenylyl phosphoramidate. Alkaline phosphatase (Escherichia coli) cleaves the terminal phosphate of the analog, a finding which extends the substrate specificity of this enzyme to include P-N-type bonds. Snake venom
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