Article Cite This: J. Phys. Chem. B 2018, 122, 6763−6770
pubs.acs.org/JPCB
Amphiphilic Ionic Liquid-Induced Membrane Permeabilization: Binding Is Not Enough Sandeep Kumar,†,§ Holger A. Scheidt,‡,§ Navleen Kaur,† Anupreet Kaur,† Tejwant S. Kang,*,† Daniel Huster,*,‡ and Venus S. Mithu*,† †
Department of Chemistry, Guru Nanak Dev University, Amritsar 143005, India Institute for Medical Physics and Biophysics, Leipzig University, Leipzig 04109, Germany
‡
J. Phys. Chem. B 2018.122:6763-6770. Downloaded from pubs.acs.org by UNIV OF GOTHENBURG on 01/23/19. For personal use only.
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ABSTRACT: The interaction of amphiphilic ionic liquids containing an 1-alkyl-3methylimidazolium cation ([C12MIM]+), which shows acute cytotoxicity toward marine and bacterial life, with zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3phospho-choline (POPC) and anionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphorac-glycerol (POPG) membranes was investigated. Biophysical parameters of this interaction were quantified by fluorescence spectroscopy, isothermal titration calorimeter, and solution-state NMR measurements. [C12MIM]+ inserts into the membrane and induces vesicle leakage at relatively low concentration (
