Subscriber access provided by UNIV OF MISSISSIPPI
Article
Analysis of Phthalate Esters in Mammalian Cell Culture Using a Microfluidic Channel Coupled with an Electrochemical Sensor Hui-Bog Noh, Nanjanagudu Ganesh Gurudatt, Mi-Sook Won, and Yoon-Bo Shim Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.5b00358 • Publication Date (Web): 19 Jun 2015 Downloaded from http://pubs.acs.org on June 23, 2015
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
Analytical Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 27
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
Analytical Chemistry
Analysis of Phthalate Esters in Mammalian Cell Culture Using a Microfluidic Channel Coupled with an Electrochemical Sensor Hui-Bog Noh,† Nanjanagudu Ganesh Gurudatt,† Mi-Sook Won,‡ and Yoon-Bo Shim*,†
†
Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan
National University, Busan 609-735, Republic of Korea. ‡
Busan Center, Korea Basic Science Institute, Busan 609-735, Republic of Korea.
[*]
Corresponding author, Prof. Yoon-Bo Shim Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, Republic of Korea. Tel.: +82-51-510-2244; Fax: +82-51-514-2122. E-mail:
[email protected] 1 ACS Paragon Plus Environment
Analytical Chemistry
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
ABSTRACT: An analytical tool to monitor trace phthalate was developed using a microfluidic channel device coupled with a novel electrochemical biosensor. At first, the electrochemical sensor was constructed with biomimetic layers to reveal a large hydrogen over potential by controlling the surface charge and hydrophobicity through assembling with a lipid (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) and a cationic molecule (toluidine blue O) bonded to a conductive polymer. The modified electrode possessing a highly negative polarization potential (approximately -1.8 V vs. Ag/AgCl) can uptake sparingly soluble phthalate ester (PEs) compounds in aqueous media. Each sensor probe material was characterized employing SEM, AFM, XPS, QCM, TEM, UVvisible, and impedance spectroscopy. The microfluidic channel is used firstly to concentrate and separate trace amount of phthalates, then the sensor probe is installed at the end of channel. Experimental variables affecting the PEs analysis were assessed and optimized in terms of biomimetic layer composition and analytical conditions. The linear dynamic range and detection limits of the PEs were 0.15 nM - 10.0 µM and ~12.5 pM with relative standard deviations
