Analysis of Phthalate Esters in Mammalian Cell Culture Using a

Jun 19, 2015 - An analytical tool to monitor trace phthalate was developed using a microfluidic channel device coupled with a novel electrochemical bi...
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Analysis of Phthalate Esters in Mammalian Cell Culture Using a Microfluidic Channel Coupled with an Electrochemical Sensor Hui-Bog Noh, Nanjanagudu Ganesh Gurudatt, Mi-Sook Won, and Yoon-Bo Shim Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.5b00358 • Publication Date (Web): 19 Jun 2015 Downloaded from http://pubs.acs.org on June 23, 2015

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Analytical Chemistry

Analysis of Phthalate Esters in Mammalian Cell Culture Using a Microfluidic Channel Coupled with an Electrochemical Sensor Hui-Bog Noh,† Nanjanagudu Ganesh Gurudatt,† Mi-Sook Won,‡ and Yoon-Bo Shim*,†



Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan

National University, Busan 609-735, Republic of Korea. ‡

Busan Center, Korea Basic Science Institute, Busan 609-735, Republic of Korea.

[*]

Corresponding author, Prof. Yoon-Bo Shim Department of Chemistry and Institute of Biophysio Sensor Technology (IBST), Pusan National University, Busan 609-735, Republic of Korea. Tel.: +82-51-510-2244; Fax: +82-51-514-2122. E-mail: [email protected]

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Analytical Chemistry

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ABSTRACT: An analytical tool to monitor trace phthalate was developed using a microfluidic channel device coupled with a novel electrochemical biosensor. At first, the electrochemical sensor was constructed with biomimetic layers to reveal a large hydrogen over potential by controlling the surface charge and hydrophobicity through assembling with a lipid (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) and a cationic molecule (toluidine blue O) bonded to a conductive polymer. The modified electrode possessing a highly negative polarization potential (approximately -1.8 V vs. Ag/AgCl) can uptake sparingly soluble phthalate ester (PEs) compounds in aqueous media. Each sensor probe material was characterized employing SEM, AFM, XPS, QCM, TEM, UVvisible, and impedance spectroscopy. The microfluidic channel is used firstly to concentrate and separate trace amount of phthalates, then the sensor probe is installed at the end of channel. Experimental variables affecting the PEs analysis were assessed and optimized in terms of biomimetic layer composition and analytical conditions. The linear dynamic range and detection limits of the PEs were 0.15 nM - 10.0 µM and ~12.5 pM with relative standard deviations