Application of Fluorescence Correlation Spectroscopy to the

Feb 9, 2007 - obtained by FCS, the relationship between temperature rise and the incident NIR laser power, ΔT/ΔP, were determined to be 62 ( 6, 49 ( 7...
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J. Phys. Chem. B 2007, 111, 2365-2371

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Application of Fluorescence Correlation Spectroscopy to the Measurement of Local Temperature in Solutions under Optical Trapping Condition Syoji Ito,* Takashi Sugiyama, Naoki Toitani, Genki Katayama, and Hiroshi Miyasaka* DiVision of Frontier Materials Science, Graduate School of Engineering Science and Center for Quantum Materials Science under Extreme Conditions, Osaka UniVersity, Toyonaka, Osaka 560-8531, Japan ReceiVed: August 10, 2006; In Final Form: NoVember 21, 2006

Fluorescence correlation spectroscopy (FCS) was applied to the quantitative evaluation of the local heating in small domains