In the Classroom
-Oxidation Wordsearch Terry L. Helser Department of Chemistry, SUNY College at Oneonta, Oneonta, NY 13820-4015;
[email protected] This puzzle contains 36 names, terms, and acronyms that describe lipid metabolism. Find and highlight these terms in the matrix below. Then, correctly transfer them to the blanks in the description beside the matrix. “Oxidation” is already done for you. Use the letters remaining in the matrix to complete the sentence describing its function. Your success will be rewarded. The answer to β-Oxidation Wordsearch is on page 518. Good hunting!
F L D S C P E N I T I N R A C
A Y E L E C T R O N Z E N N Y
F C H K E T O A C Y L O H O C
T A Y Y I O E A E G I L D I L
H Y D R O X Y B U T Y R A T E
I X R I D I S E A O C O N A S
O O O A C D E V N I C P C V A
L R G Y O A I E B O D E M R T
A D E T A T E C A O T E C A A
S Y N T C I N S R O D E S T R
E H A A Y O H P N I C I K S D
T T S F L N T E H D A F E A Y
K R E B S A C E T Y L U N S H
The β-O X I D A T I O N of a F _ _ _ _ _ _ _ _ begins with its A _ _ _ _ _ _ _ _ _ in a cell’s cytoplasm. _ _ _ hydrolysis drives the addition of a C _ _ _ _ to form _ _ _ and an A _ _ _-_ _ _. C _ _ _ _ _ _ _ _ transports it into the mitochondrial matrix where F _ _ oxidizes it to E _ _ _ __ _ _. Then a H _ _ _ _ _ _ _ _ adds water to make an L-βH _ _ _ _ _ _ _ _ _ _-_ _ _. A D _ _ _ _ _ _ _ _ _ _ _ _ uses _ _ _+ to oxidize this to a β-K _ _ _ _ _ _ _-_ _ _. Finally, T _ _ _ _ _ _ _ cleaves it, making a shorter acyl-CoA that recycles, and an A _ _ _ _ _-_ _ _. This drives the K _ _ _ _ C _ _ _ _, and the _ _ _ _ 2 and N _ _ _ produced here and above drive E _ _ _ _ _ _ _ transport making lots of _ _ _, which is the point, after all. Abnormal states like D _ _ _ _ _ _ _ and S _ _ _ _ _ _ _ _ _ lead to ketosis, in which the K _ _ _ _ _ B _ _ _ _ _, A _ _ _ _ _ _ _ _ _ _ _, 3-H _ _ _ _ _ _ _ _ _ _ _ _ _ _, and A _ _ _ _ _ _, are made.
Use the remaining 20 letters to fill in the following sentence: _ _ _ _ _
_ _ _ _ _
_ _ _ _ _ _ _ _
JChemEd.chem.wisc.edu • Vol. 78 No. 4 April 2001 • Journal of Chemical Education
_ _.
483
In the Classroom
Answers to the -Oxidation Wordsearch on page 483
F L D S C P E N I T I N R A C
A Y E L E C T R O N Z E N N Y
F C H K E T O A C Y L O H O C
T A Y Y I O E A E G I L D I L
H Y D R O X Y B U T Y R A T E
I X R I D I S E A O C O N A S
O O O A C D E V N I C P C V A
L R G Y O A I E B O D E M R T
A D E T A T E C A O T E C A A
S Y N T C I N S R O D E S T R
E H A A Y O H P N I C I K S D
T T S F L N T E H D A F E A Y
K R E B S A C E T Y L U N S H
The β-OXIDATION of a FATTY ACID begins with its ACTIVATION in a cell’s cytoplasm. ATP hydrolysis drives the addition of a COASH to form AMP and an ACYL-COA. CARNITINE transports it into the mitochondrial matrix where FAD oxidizes it to ENOYL-COA. Then a HYDRATASE adds water to make an L-β-HYDROXYACYLCOA. A DEHYDROGENASE uses NAD+ to oxidize this to a β-KETOACYL-COA. Finally, THIOLASE cleaves it, making a shorter acyl-CoA that recycles, and an ACETYLCOA. This drives the KREBS CYCLE, and the FADH2 and NADH produced here and above drive ELECTRON transport making lots of ATP, which is the point, after all. Abnormal states like DIABETES and STARVATION lead to ketosis, in which the KETONE BODIES, ACETOACETATE, 3HYDROXYBUTYRATE, and ACETONE, are made. FATTY ACIDS ENERGIZE US.
518
Journal of Chemical Education • Vol. 78 No. 4 April 2001 • JChemEd.chem.wisc.edu
