Biobased and Aromatic Reversible Thermoset Networks from

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Biobased and aromatic reversible thermoset networks from condensed tannins via the Diels-Alder reaction Antoine Duval, Guillaume Couture, Sylvain Caillol, and Luc Averous ACS Sustainable Chem. Eng., Just Accepted Manuscript • DOI: 10.1021/ acssuschemeng.6b02596 • Publication Date (Web): 07 Dec 2016 Downloaded from http://pubs.acs.org on December 11, 2016

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ACS Sustainable Chemistry & Engineering

Biobased and aromatic reversible thermoset networks from condensed tannins via the DielsAlder reaction Antoine Duval,1 Guillaume Couture,2 Sylvain Caillol,2 Luc Avérous1* 1

BioTeam/ICPEES-ECPM, UMR CNRS 7515, Université de Strasbourg, 25 rue Becquerel, 67087

Strasbourg, Cedex 2, France. 2

Institut Charles Gerhardt, Montpellier, UMR 5253 - CNRS, Université Montpellier, ENSCM - 8 rue

de l’Ecole Normale, 34296 Montpellier Cedex 5, France. * Corresponding author: Prof. Luc Avérous, Phone: + 333 68852784, Fax: + 333 68852716, Email: [email protected]

1 ACS Paragon Plus Environment


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Abstract Thermo-reversible networks were obtained for the first time from tannins, an aromatic biobased polyphenol, by reacting furan-bearing tannins and telechelic oligomers with maleimide end groups, using the Diels-Alder (DA) reaction. Condensed tannins from mimosa (Acacia mearnsii) were functionalized with furfuryl glycidyl ether and thoroughly characterized by 1H,

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P NMR and FTIR

spectroscopy. The accessibility of the grafted furan groups was confirmed by a model reaction with Nmethylmaleimide. Different crosslinked networks were then obtained by DA reaction with three PPO and PPO-b-PEO-b-PPO oligomers, and evidenced by FTIR spectroscopy. The thermal properties of the obtained networks were evaluated with differential scanning calorimetry (DSC) and thermogravimetric analysis. Then, the reversibility of the crosslinking was shown by a quick return to the liquid state upon heating at 120 °C. The retro Diels-Alder reaction was studied by size exclusion chromatography and DSC. Keywords: Tannins, Diels-Alder, retro Diels-Alder, Crosslinking, biobased


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Introduction With the increasing lack of certain fractions from fossil resources, the subsequent price volatility, and an increasing pressure from the civil society for a more sustainable industry, researchers, and among them chemists, are urged to find alternatives to fossil-based derivatives. Biobased aromatic derivatives are especially sought, since BTX (Benzene, Toluene, Xylene) fractions from naphta are nowadays less and less available due to the strong increase of the global demand. However, aromatic monomers are largely used in polymer materials for their rigidity, chemical and thermal stability, as well as their ability to structure the matter by π-stacking, thus providing high performances. Finally, biobased aromatic derivatives can also bring some new chemical architectures. Among the aromatic biobased compounds, tannins appear as interesting candidates in the field of materials chemistry. After lignins, they are the second most abundant natural source of phenolic derivatives.1 They are found in the soft tissues (sheets, needles or bark) of all vascular plants and exhibit various structures divided into three main families: hydrolyzable, complex, and condensed tannins.2 The latter represents 90% of the world production, the large majority being extracted from Acacia mearnsii (commonly known as mimosa or black wattle).3 Condensed tannins are based on four types of repeating flavonoid units: profisetinidin, procyanidin (catechin), prorobinetinidin, and prodelphinidin, linked by C4-C6 or C4-C8 bonds.4,5 Thanks to their specific aromatic structure, they could provide high thermal and chemical stability, as well as toughness, while the numerous phenolic functions and nucleophilic sites may easily lead to various functionalizations.1 For all these reasons, tannins or their depolymerized building units have been successfully used in the synthesis of polyols,6– 8

epoxy resins,9–13 acrylate resins,14,15 adhesives1 or foams.16–20

An important concern in polymer science, especially with thermosets, is to obtain reversible crosslinking bonds. Indeed, chemically crosslinked networks cannot be recycled by heating. Thus, a reversible crosslinking that allows depolymerizing the network is a desirable feature for an easy valorization at the end of life of the materials.21 It may also be a route toward stimuli-responsive, 3 ACS Paragon Plus Environment


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shape memory or self-healing materials.22–25 Various parameters have been used to trigger the crosslinking/un-crosslinking reactions such as temperature, radiations, pH or processing.26 Among them, temperature is often seen as the easiest to apply, especially for industrial applications.27 Several systems can be used to form reversible covalent bonds with temperature including urethane or ester bonds, nitroso or azlactone groups, ionene formation and Diels-Alder reaction. The Diels-Alder (DA) [4 + 2] cycloaddition involves a diene and a dienophile reacting to yield a substituted cyclohexene, which can be broken by further increasing the temperature via the retro Diels-Alder reaction (rDA).28,29 The furan-maleimide system exhibits relatively low reaction temperature for DA and rDA, around 60-70 °C and 110-120 °C, respectively, making it compatible with the thermal stability of most polymeric materials.30 In this study, we first designed original furan-bearing tannins via the reaction of mimosa (Acacia mearnsii) tannins with furfuryl glycidyl ether in various ratios. These new functionalized tannins were characterized by spectroscopic techniques (1H,

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P NMR and FTIR), and size exclusion

chromatography (SEC). Then, the accessibility of the grafted furan groups was evidenced by the study of the DA and rDA reactions with N-methylmaleimide. Original biobased networks were finally prepared with three different bismaleimide crosslinkers based on telechelic oligo(propylene oxide) and oligo(ethylene oxide). The efficient synthesis of the DA adducts and the reversibility of the networks was finally evidenced by different techniques such as 1H NMR spectroscopy at high temperature, FTIR, SEC and differential scanning calorimetry (DSC).

Experimental section Materials Condensed tannins from mimosa (MT, Acacia mearnsii, Fintan OP®) were kindly supplied by Silva Chimica (St. Michele Mondovi, Italy). Prior to analysis, they were dried in an oven at 70 °C for one night, and stored in a desiccator. Pyridine (sequencing grade, ≥ 99.5 %) was purchased from Fisher Scientific, acetic anhydride (ACS reagent, ≥ 97%) and furfuryl glycidyl ether (FGE, 97%) from Acros Organics, and methanol (laboratory reagent grade, ≥ 99.6 %), dichloromethane (puriss., ≥ 99 %), 4 ACS Paragon Plus Environment

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2,3,4,5,6-pentafluorobenzaldehyde (98%), N-methyl maleimide (97 %), 2-chloro-4,4,5,5-tetramethyl1,3,2-dioxaphospholane (95%), dimethylsulfoxide (≥ 99.9 %), DMSO-d6 (99.9 % D atoms) and CDCl3 (99.8 % D atoms) from Sigma-Aldrich. The crosslinkers poly(propylene oxide) bismaleimide (DP = 3 or 6) and poly(propylene oxide)-b-poly(ethylene oxide)-b-poly(propylene oxide) bismaleimide were purchased from Specific Polymers (Castries, France). All chemicals were used as received without further purification. Syntheses Reaction of tannins with furfuryl glycidyl ether (FGE) Mimosa tannins (MT) were dissolved in water containing NaOH (1 molar equivalent to total phenolic OH in tannins). After 30 min stirring, FGE (from 0.25 to 1.5 molar equivalents to total phenolic OH in tannins) was added dropwise and the reaction mixture was stirred overnight at room temperature. The solution was then acidified to precipitate the functionalized tannins, down to pH ranging from 5 to 1. The precipitate was recovered by centrifugation, washed 3 times with 50 mL of acidified water (same pH as the precipitation pH), and freeze-dried. Acetylation Acetylation was performed in a pyridine/acetic anhydride mixture (1:1 v/v), as previously reported on tannin samples.6 DA reaction with N-methyl maleimide In a 10 ml round-bottom flask, 0.1 g of furan-bearing MT was dissolved in 2 g of DMSO. After adding 31.2 mg of N-methyl maleimide, the mixture was heated at 70 °C for 15 days under magnetic stirring. The crude product was cooled down, precipitated in 40 ml of toluene and centrifuged at 3000 rpm for 3 min. After removing the liquid phase, the brown solid was dried overnight in an oven at 40 °C under vacuum (10-2 mbar). DA and rDA with bismaleimide linkers In a small flask, 0.1 g of furan-containing mimosa tannins was dissolved in DMSO at 10 wt.% and a precise amount of bis-maleimide crosslinker was added. After vigorous stirring, the mixture was 5 ACS Paragon Plus Environment


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heated at 70 °C in an oven. The crosslinking reaction was checked daily by turning the flask upsidedown to control the gel formation. The rDA reaction was carried out by heating the crosslinked sample at 120 °C in an oven for 1 hour, thus returning the mixture to its liquid state. Characterizations FTIR spectroscopy FTIR spectrum were recorded in the attenuated total reflectance (ATR) mode on a Nicolet 380 FTIR or a Perkin-Elmer Spectrum 1000 spectrometer, in the range 400–4000 cm-1, as the average of 32 scans with 4 cm-1 resolution. The samples were deposited on the ATR crystal and carefully pressed to ensure a good contact. The background was recorded with the empty ATR crystal in air. Size Exclusion Chromatography (SEC) SEC measurements on furan-bearing tannins were performed in chloroform (HPLC grade) in a Shimadzu liquid chromatograph equipped with a 5µ PL Gel Guard column, two PL-gel 5µ MIXED-C and a 5µ 100 Å 300 mm-columns, and 2 online detectors, a Shimadzu RID-10A refractive index detector and a Shimadzu SPD-M10A diode array (UV) detector. Molar masses and dispersity were calculated from a calibration with polystyrene standards. Acetylated tannin samples were dissolved in chloroform and filtered through a 0.2 µm PTFE membrane. For all analyses the injection volume was 50 µL, the flow rate 0.8 mL min-1 and the oven temperature set at 25 °C. SEC evidencing the rDA reaction were recorded using a triple detection SEC from Agilent Technologies. The system used two PL1113-6300 ResiPore 300 × 7.5 mm columns with DMF as the eluent at a flow rate of 0.8 mL min-1. The detector suite comprised a PL0390-0605390 LC light scattering detector with 2 diﬀusion angles (15 ° and 90 °), a PL0390-06034 capillary viscometer, and a 390-LC PL0390-0601 refractive index detector. The entire SEC system was thermostated at 35 °C. PMMA standards were used for the calibration. 1

H NMR

The spectra were acquired on a Bruker 400 MHz spectrometer (10 s delay, 32 scans) at room temperature or 100 °C for the rDA characterization. An accurately weighed amount of sample (about 6 ACS Paragon Plus Environment

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20 mg) was dissolved in 500 µL of deuterated solvent. For the neat samples, DMSO-d6 was used, whereas the acetylated tannins were analyzed in CDCl3. For NMR titration, a precise amount of sample and external standard were weighed: 2,3,4,5,6-pentafluorobenzaldehyde or 2,4,6trimethylphenol for furan or maleimide determination, respectively. 31

P NMR

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P NMR was performed after phosphitylation of the samples, according to standard protocols.31 The

spectra were measured on a Bruker 400 MHz spectrophotometer, with 128 scans and a 15 s relaxation delay. Peaks assignations were performed based on previous studies on tannin model compounds.32,33 Thermal characterizations Thermogravimetric analyses (TGA) were carried out on a TGA 51 apparatus from TA Instruments, from room temperature to 700 °C, at a heating rate of 10 °C min-1 under nitrogen flow. Diﬀerential scanning calorimetry (DSC) analyses were performed under an inert atmosphere with a calorimeter DSC1 from Mettler Toledo. All the samples were heated from − 60 to 190 °C at a heating rate of 10 °C min-1, then cooled down to room temperature for 1 h prior to a second run.

Results and discussion Grafting of furan groups onto mimosa tannins (MT) MT were reacted with FGE to obtain a multifunctional furan-containing polymer (Scheme 1). The reaction was conducted with various amounts of FGE, ranging from 0.25 to 1.5 equivalents to total phenolic OH in tannins, and the product was recovered and purified after an acidic precipitation. The reaction consumes phenolic OH groups and creates new aliphatic OH groups, as a result of the opening of the oxirane ring. Similar reaction conducted on lignin showed that under these reaction conditions only secondary aliphatic OH groups are created,34 i.e. that the nucleophilic attack of the epoxy ring by the phenolate anion only takes place at the less hindered carbon.



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Scheme 1. Reaction of tannins with furfuryl glycidyl ether (FGE).

The content in OH of the modified tannins was measured by 31P NMR on phosphitylated samples and 1

H NMR on acetylated samples (Figure 1a). Figure 1 shows the content in phenolic and aliphatic OH

groups depending on the amount of FGE used for the reaction. The decrease in phenolic groups attests for the successful grafting (Figure 1b). Surprisingly, the content in aliphatic OH groups does not increase significantly with the grafting (Figure 1c). The aliphatic OH content is even lower for the derivatized tannins than for the initial one. A careful study of the 31P NMR spectra of the initial tannin, shown in the supporting information (Figure S1), reveals the presence of carbohydrate impurities, which give rise to some signal in the 147 – 150 ppm region.8 After the reaction, this signal disappeared, suggesting that the residual carbohydrates were lost, probably during the washing steps in water. A new well-defined peak at 146.3 ppm confirms the creation of the new aliphatic OH group, generated by the opening of the epoxy ring (Scheme 1).


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Figure 1. Derivatization of OH groups with 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane (top) or acetic anhydride (bottom) for the quantification by 31P or 1H NMR, respectively (a), and quantity of phenolic (b) and aliphatic OH groups (c) depending on the amount of FGE used for the reaction.

Surprisingly, the values obtained with 31P NMR are always significantly lower than those measured by 1

H NMR (Figure 1b and c), whereas previous studies showed a good correlation between both

measurements in condensed tannins.35 Both measurements rely on the derivatization of the remaining OH groups, either with a phospholane or an acetyl group. As shown on Figure 1a, the phospholane group is much bulkier than the acetyl group, and it thus seems possible that it fails to access all of the remaining OH groups present in the derivatized tannins, leading to an underestimation. The precise quantification of the grafted furan groups is mandatory to study the DA reaction. It can directly be obtained from the decrease in phenolic OH groups ∆(φ-OH), taking into account the mass increase induced by the grafting (Equation 1):

(1) where ∆(φ-OH) stands for the decrease in phenolic OH groups (in mol g-1) and MFGE for the molar mass of FGE (154.2 g mol-1). The calculation has been performed taking into account the ∆(φ-OH) value obtained by 1H NMR. The results are compiled in Table 1. 1H NMR was also used to directly quantify the amount of furan groups grafted to the tannins. The H atoms from the furan ring give rise to two strong signals at respectively



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6.4 (2H) and 7.6 ppm (1H) (Figure 2a). The latter was used to quantify the amount of furan groups present in each sample, since it is fairly well separated from the aromatic H atoms of tannins. A good correlation between both quantification techniques is obtained. The amount of grafted furan groups quickly rises with the amount of FGE used, to reach a plateau for more than 0.75 eq. Table 1. Content in OH and furan groups in the derivatized tannins measured with 31P and 1H NMR.

Name

FGE eq.

MT MT-F0.25 MT-F0.5 MT-F0.75 MT-F1 MT-F1.25 MT-F1.5

0 0.25 0.5 0.75 1 1.25 1.5

Residual φ-OH (mmol g-1)a 3.72 2.76 2.43 2.29 2.02 1.41

Furan content (mmol g-1) Average By direct 1H By ∆(φ-OH) c b NMR method method 2.12 2.23 2.18 ± 0.05 2.52 2.63 2.58 ± 0.05 2.64 3.22 2.93 ± 0.29 2.69 3.34 3.02 ± 0.33 2.78 3.09 2.94 ± 0.16 2.97 2.96 2.97 ± 0.01

a

Based on 1H NMR data Determined using Equation 1 c Obtained by direct integration of furan signal in 1H NMR (Figure 2) b


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Figure 2. Detail of the 1H NMR spectra of the initial tannin (MT) and tannin derivatized with 1 equivalent FGE (MTF1) (a), and FTIR spectra of the same (b).

FTIR was also used to evidence the successful grafting (Figure 2b). New characteristic signals assigned to the grafted FGE appear clearly: the C-O stretch around 1070 cm-1, or the furan ring at 740 cm-1. The height of this last peak is a good indicator of the grafting, since it is proportional to the content in furan groups measured by 1H NMR (Figure S2 in SI). In addition, a signal is detected in the C=O stretch region around 1710 cm-1. It was later found that its intensity increased linearly when the precipitation pH was reduced (Figure S3 in SI), thus suggesting acid-induced side reactions occurring during the precipitation and/or washing steps. A similar band was observed on the neat tannin after solubilizing in water, acidifying to pH 2 and freeze-drying (Figure S4 in SI). This suggests that the side reaction affects the tannin backbone rather than the grafted side chain (a putative mechanism is proposed in the SI, Scheme S1).



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Precipitation and washings should then be conducted at an intermediate pH to avoid important changes in the tannin structure. However, since the recovered yield is also correlated to the precipitation pH (Figure S5 in SI), a compromise has to be found. For the rest of the study, a precipitation at pH of 3 was chosen. SEC was then performed on all samples. The chromatograms are presented on Figure 3a. The derivatization causes a clear shift toward higher molar masses. The positions of the two main peaks then remain unchanged, whatever the amount of FGE used for the reaction. Increasing signal in the high molar mass region suggests possible crosslinking reactions, leading to large increase in the weight-average molar mass Mw (Figure 3b).

Figure 3. SEC of the derivatized tannins (a) and evolution of Mn and Mw with the amount of FGE used for the reaction (b)

All these results show that using a large excess of FGE is not necessary, since it doesn’t allow to significantly increase the grafting (Table 1), nor to ameliorate the recovered yield (Figure S6 in SI). For the rest of the study, MT modified with 1.15 eq. of FGE was used unless stated otherwise.


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Model study of DA and rDA reaction with N-methylmaleimide To study the DA reaction with furan-bearing tannins, a model reaction was carried out with N-methyl maleimide (Scheme 2). This compound has been chosen as the expected NMR signal of the methylic protons for the synthesized DA adduct will not overlap with other tannin signals.

Scheme 2. Reaction of furan-bearing tannins with N-methyl maleimide. R1 stands for -CH2-CH(OH)-CH2-furan and R2 stands for -CH2-CH(OH)-CH2-DA Adduct.

After purification, the tannin reacted with N-methyl maleimide was characterized by 1H NMR spectroscopy (Figure 4). The success of the model reaction was confirmed by the appearance of characteristic signals of the protons of a Diels-Alder adduct at 2.9-3.0 ppm (signals k, j and j’), 3.7-4.2 ppm (d’) and 6.5 ppm (g and g’) as well as the decrease of the signals of the furan ring at 6.4 and 7.6 ppm (e and e’, and f, respectively). However, the latter did not completely disappear indicating that the reaction is not complete. The reaction time was increased up to 15 days and the remaining furan groups were titrated via NMR by comparing signal f with 2,3,4,5,6-pentafluorobenzaldehyde as an external standard. The conversion was found to be of 50 %, the DA reaction being limited, for instance by steric hindrance.



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Figure 4. 1H NMR spectra of furan-functionalized mimosa tannins (upper spectrum) and furan-functionalized mimosa tannins reacted with N-methyl maleimide (lower spectrum) recorded in DMSO-d6. R1 = -CH2-CH(OH)-CH2furan, R2 = -CH2-CH(OH)-CH2-DA Adduct.

rDA reaction was monitored by 1H NMR at 100 °C by recording spectra every 20 minutes for 3 hours. Figure 5 displays the spectra recorded at t0 and after three hours of heating. Several significant changes can be observed including the increase of the signals at 6.3 and 7.5 ppm (e, e’ and f) corresponding to the protons of the furan ring, and the decrease of the signals between 2.6 and 3.0 ppm (j and j’), at 3.7 and 4.2 ppm (d’), and at 6.5 ppm (g and g’) related to the Diels-Alder adduct. The 3 hours heating at 100 °C were not sufficient to yield the fully “regenerated” furan-bearing tannins, as some signals from Diels-Alder adduct are still visible, for example at 6.5 ppm.


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Figure 5. 1H NMR spectra recorded at 100 °C in DMSO d6 for the rDA reaction of furan-bearing mimosa tannins functionalized with N-methyl maleimide, at the beginning of the reaction (lower spectrum) and after three hours (upper spectrum).

Crosslinking of furan-bearing tannins with bismaleimide oligomers Three bismaleimide linkers were used to crosslink the furan-bearing tannins (Scheme 3). Crosslinkers X1 and X2 are telechelic poly(propylene oxide) of different chain lengths, whereas X3 is based on a poly(propylene oxide)-b-poly(ethylene oxide)-b-poly(propylene oxide) chain. The average degree of polymerization and precise content in maleimide groups of the crosslinkers have been determined by 1

H NMR (Appendix A in SI). The results are gathered in Table 2.

Scheme 3. Chemical structures of the maleimide-terminated telechelic oligomers used as crosslinkers.



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Table 2. Degree of polymerization and maleimide content of the bismaleimide crosslinkers.

Degree of polymerizationa

X1 X2 X3 a

Maleimide (mmol.g-1) 5.00 ± 0.10 3.09 ± 0.04 1.60 ± 0.04

n

x+z

y

3.9 7.9 -

3.9

17.9

See Scheme 3 for the meaning of n, x, y and z.

Functionalized tannins were crosslinked with the three bismaleimide oligomers in a 1:1 maleimide/furan molar ratio with the same experimental conditions (Samples XMT1, XMT4 and XMT5 in Table 3). The furan molar ratio was also increased to 1.29 and 1.73 to determine its influence on the final properties of the crosslinked networks (Samples XMT1, XMT2 and XMT3 in Table 3). The DA reaction was carried out at 70 °C in an oven (Figure 6) and checked regularly by returning bottles (Pictures a and b). After 3 days, XMT5 was already crosslinked, whereas XMT3 and XMT4 were only partially crosslinked. The presence of a long PEO block in the middle of crosslinker X3 brings additional flexibility to the chains, which may facilitate the reaction between the terminal maleimide groups and the furans. All samples were crosslinked gels after 7 days (Picture c). Table 3. Crosslinking experimental conditions for the functionalized tannins and thermal properties of the obtained networks.

TGA Entry XMT1 XMT2 XMT3 XMT4 XMT5 a b

Crosslinker X1 X1 X1 X2 X3

Maleimide: furan ratio 1:1.02 1:1.29 1:1.73 1:1.06 1:1.04

Td,10% (°C) 298 304 256 293 347

Tmax (°C) 400 403 408 394 399

Char (%)

Tg (°C)

37 35 37 30 23

n.o. n.o. n.o. n.o. 4

a

DSC TrDA-endo (°C)b 126 133 129 127 125

TrDA-exo (°C)b 158 159 156 155 155

Determined by DSC during the first heating ramp. n.o. = not observed Determined by DSC during the second heating ramp.


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Figure 6. Pictures of crosslinked and uncrosslinked furan-bearing tannins solutions according to the applied temperature profile.

rDA reaction was then carried out by heating the gels at 120 °C in an oven. After 1 hour at this temperature, all samples returned back to their liquid state (Picture d on Figure 6). The reversibility of the system was tested by heating the solutions at 70 °C again. The crosslinking reaction occurred more rapidly as all samples were back to the gel state after only 2 days (Picture e), probably because the previous rDA reaction was not complete. After crosslinking in solution, the gels were washed with water to remove the DMSO and dried under vacuum. They were then analyzed by FTIR and compared with both the furan-bearing tannins and the crosslinkers. Results obtained for sample XMT5 are given in Figure S7 in SI, as a representative example. The DA reaction was confirmed by the disappearance of the furan ring characteristic signals: the out-of-plane bending of the C-H bond at 738 cm-1 and the stretching of the C=C double-bond at 1459 cm-1,36 as well as those of the maleimide ring: the ring bending at 694 cm-1, the C-H bond bending at 830 cm-1 and the C=C double-bond stretching at 1639 cm-1.37 Moreover, the crosslinked tannins combine both the stretching signals of O-H, C-H, C=O and C=C bonds brought by the tannins and the crosslinker X3. Other entries exhibit similar spectra indicating a successful crosslinking for all samples. Thermal properties of the networks were then evaluated with TGA and DSC. The thermal stability was first evaluated with TGA. The weight loss and derivative weight loss vs. temperature curves are 17 ACS Paragon Plus Environment


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displayed on Figure 7, and the maximum degradation temperatures and char amounts are gathered in Table 3. All materials exhibit an important one-step weight loss with a maximum degradation around 400 °C, caused by the degradation of the ether groups of PEO and PPO chains of the crosslinker, as well as FGE chains. Crosslinked materials XMT1 to XMT4 also have a small weight loss below 300 °C, which probably arises from the evaporation of residual water and DMSO entrapped within the polymeric network. The high crosslink density of these networks, caused by the short PPO chains (Table 2), probably caused the incomplete removal of the solvents during the drying step. On the contrary, sample XMT5 does not exhibit any degradation before 300 °C, thus indicating a very dry material because of the longer crosslinker chains, which allow an easy removal of solvents during vacuum drying.

Figure 7. Mass % vs. Temperature (left) and Weight derivative curves vs. Temperature (right) for crosslinked networks XMT1 to XMT5. Thermograms were recorded at 10 °C min-1 under nitrogen.

A high char amount (from 23 to 37 %) is obtained because of the stability of the phenolic tannin backbone, being greater for example than a crosslinked epoxy resin based on green tea tannin (21 % of char at 580 °C under similar conditions).10 The char content decreases when increasing the crosslinker chain length as the relative content of aliphatic units in the materials is higher. Surprisingly, increasing the amount of crosslinker has little influence on the char amount (Samples XMT1 to XMT3, Table 3), whereas decreasing char amounts were expected as a result of a higher aliphatic content.


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Figure 8. DSC thermograms of XMT5 recorded under N2 from -60 °C to 190 °C at a heating rate of 10 °C/min. The first heating step is displayed in green, the second heating is displayed in black.

The networks were then analyzed by DSC, and representative results obtained on sample XMT5 are displayed on Figure 8. During the first heating ramp, DSC thermograms exhibit two endothermic peaks around 130 and 160 °C corresponding to the rDA reaction for endo and exo adducts, respectively,38 as endo adduct undergoes rDA at lower temperature.39 The temperatures of the rDA for endo and exo adducts are reported on Table 3. They were found to be independent of the crosslinker (128 ± 3 °C for endo and 157 ± 2 °C for exo). After 1 h cooling, a second heating ramp yields peaks with similar shape around the same temperatures, arising from an incomplete rDA reaction during the first heating step. This phenomenon is confirmed by the absence of significant exothermic signal around 70 °C during the first cooling and second heating steps, indicating that no DA adducts are formed back. Futhermore, if temperature is kept at 150 °C for 4 hours after the first heating step, remaining DA adducts are still observed through the characteristic endothermic peaks during the second heating. However, the reversibility of the crosslinking was confirmed by keeping the sample at 70 °C for 6 hours prior to a third heating ramp. In fact, the area of the rDA peaks was found to be higher for this last step than during the second heating step, showing that adducts were formed through DA reaction (Figure S8 in SI). The sample prepared with the longest crosslinker XMT5, is the only one to exhibit a glass transition temperature (Tg). The pristine crosslinker exhibited a Tg of -53 °C, whereas the crosslinked network has a much higher Tg of 4 °C, as measured during the first heating ramp. This is due to the presence of 19 ACS Paragon Plus Environment


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the aromatic backbone of MT, as well as the secondary OH groups brought by the FGE ring-opening, which can allow hydrogen bonds. During the second heating ramp, the Tg is reduced to -7 °C. This decrease is probably caused by the rDA that occurred during the first heating ramp: the crosslink density is lowered, causing a reduced Tg. In addition, crosslinker molecules liberated during the rDA can act as plasticizer, thus further reducing the Tg.

Figure 9. Normalized refractive index (RI) response of SEC chromatograms of crosslinker X3 (in red), furan-bearing tannins (in blue) and crude product of XMT5 sample after rDA in DMSO. The picture on the left shows the insolubility of XMT5 in DMF prior to the rDA reaction.

The crosslinked networks are insoluble in organic solvents, as shown on Figure 9, where the sample XMT5 is immersed in a large excess of DMF. After performing the rDA during 1h at 120 °C in DMSO, the sample became soluble and could be analyzed by SEC. After the rDA, XMT5 exhibits a sharp signal around 23.5 min and a broad signal between 14 and 23 min. The first corresponds to the free crosslinker X3, while the second has a shoulder indicating the presence of the furan-bearing tannins. The broad signal from 14 to 20 min arises from an incomplete rDA reaction (as stated by DSC) leading to soluble, but still partially crosslinked oligomers, which thus exhibit higher molar mass.


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Conclusion The Diels-Alder reaction between furan and maleimide moieties was exploited to prepare new tanninbased thermo-reversible materials. In a first step, condensed tannins from mimosa (Acacia mearnsii) were modified with FGE, to expose furan groups as chain end. The reaction was confirmed by FTIR, 31

P and 1H NMR. Despite a non-quantitative reaction, derivatized tannins with a high content in furan

groups were obtained, up to 3 mmol g-1. A model study for the reaction of furan-bearing tannins with maleimides was carried out with N-methylmaleimide. The DA reaction was evidenced by 1H NMR with a conversion reaching 50 %, and its reversibility assessed with in situ 1H NMR measurements at 100 °C showing the rDA. The furan-bearing tannins were then crosslinked with 3 telechelic oligomers of PPO or PPO-b-PEO-b-PPO terminated by maleimide groups. The DA reaction, evidenced by FTIR, led to the formation of gels during storage at 70 °C. The gels were able to quickly turn back to the liquid state upon exposure to higher temperatures (120 °C), even though DSC and SEC experiments showed that the rDA reaction was not completed in such a short time. Finally, the obtained aromatic materials exhibited high thermal stabilities and char amounts. The use of these new multi-functionnal tannins and crosslinkers may open the way for tunable, partially or fully bio-based networks with a high recycling/reuse potential, for a very large range of fields such as reversible adhesive, shape memory or self-healing materials, for building, automotive or biomedical applications.

Acknowledgments Silvateam (Italy), and more particularly Dr. Samuele Giovando, is gratefully acknowledged for kindly supplying tannin samples. Chheng Ngov (ICPEES, Université de Strasbourg) is thanked for her technical support.

Supporting Information 31

P NMR and FTIR spectra of the derivatized tannins, yields of the tannin derivatization reactions,

FTIR spectra and DSC curves of the Diels-Alder crosslinked networks are available as supporting



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information.


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References (1) (2) (3)

(4) (5) (6)

(7) (8) (9)

(10)

(11) (12)

(13)

(14) (15)

(16) (17) (18) (19)

(20)

Arbenz, A.; Avérous, L. Chemical modification of tannins to elaborate aromatic biobased macromolecular architectures. Green Chem. 2015, 17 (5), 2626–2646. Quideau, S.; Deffieux, D.; Douat-Casassus, C.; Pouységu, L. Plant Polyphenols: Chemical Properties, Biological Activities, and Synthesis. Angew. Chem. Int. Ed. 2011, 50 (3), 586–621. Chan, J. M.; Day, P.; Feely, J.; Thompson, R.; Little, K. M.; Norris, C. H. Acacia mearnsii industry overview: current status, key research and development issues. South. For. J. For. Sci. 2015, 77 (1), 19–30. Khanbabaee, K.; van Ree, T. Tannins: Classification and Definition. Nat. Prod. Rep. 2001, 18 (6), 641–649. Aron, P. M.; Kennedy, J. A. Flavan-3-ols: Nature, occurrence and biological activity. Mol. Nutr. Food Res. 2008, 52 (1), 79–104. Arbenz, A.; Averous, L. Synthesis and characterization of fully biobased aromatic polyols oxybutylation of condensed tannins towards new macromolecular architectures. RSC Adv. 2014, 4 (106), 61564–61572. Arbenz, A.; Avérous, L. Oxyalkylation of gambier tannin—Synthesis and characterization of ensuing biobased polyols. Ind. Crops Prod. 2015, 67, 295–304. Duval, A.; Avérous, L. Oxyalkylation of Condensed Tannin with Propylene Carbonate as an Alternative to Propylene Oxide. ACS Sustain. Chem. Eng. 2016, 4 (6), 3103–3112. Nouailhas, H.; Aouf, C.; Le Guerneve, C.; Caillol, S.; Boutevin, B.; Fulcrand, H. Synthesis and Properties of Biobased Epoxy Resins. Part 1. Glycidylation of Flavonoids by Epichlorohydrin. J. Polym. Sci. Part -Polym. Chem. 2011, 49 (10), 2261–2270. Benyahya, S.; Aouf, C.; Caillol, S.; Boutevin, B.; Pascault, J. P.; Fulcrand, H. Functionalized green tea tannins as phenolic prepolymers for bio-based epoxy resins. Ind. Crops Prod. 2014, 53, 296–307. Aouf, C.; Benyahya, S.; Esnouf, A.; Caillol, S.; Boutevin, B.; Fulcrand, H. Tara tannins as phenolic precursors of thermosetting epoxy resins. Eur. Polym. J. 2014, 55, 186–198. Basnet, S.; Otsuka, M.; Sasaki, C.; Asada, C.; Nakamura, Y. Functionalization of the active ingredients of Japanese green tea (Camellia sinensis) for the synthesis of bio-based epoxy resin. Ind. Crops Prod. 2015, 73, 63–72. Baroncini, E. A.; Kumar Yadav, S.; Palmese, G. R.; Stanzione, J. F. Recent advances in biobased epoxy resins and bio-based epoxy curing agents. J. Appl. Polym. Sci. 2016, 133 (45), n/an/a. Jahanshahi, S.; Pizzi, A.; Abdulkhani, A.; Shakeri, A. Analysis and Testing of Bisphenol AFree Bio-Based Tannin Epoxy-Acrylic Adhesives. Polymers 2016, 8 (4), 143. Jahanshahi, S.; Pizzi, A.; Abdulkhani, A.; Doosthoseini, K.; Shakeri, A.; Lagel, M. C.; Delmotte, L. MALDI-TOF, C-13 NMR and FT-MIR analysis and strength characterization of glycidyl ether tannin epoxy resins. Ind. Crops Prod. 2016, 83, 177–185. Luo, C.; Grigsby, W. J.; Edmonds, N. R.; Al-Hakkak, J. Vegetable oil thermosets reinforced by tannin–lipid formulations. Acta Biomater. 2013, 9 (2), 5226–5233. Lacoste, C.; Basso, M. C.; Pizzi, A.; Laborie, M.-P.; Celzard, A.; Fierro, V. Pine tannin-based rigid foams: Mechanical and thermal properties. Ind. Crops Prod. 2013, 43, 245–250. Basso, M. C.; Pizzi, A.; Al-Marzouki, F.; Abdalla, S. Horticultural/hydroponics and floral natural foams from tannins. Ind. Crops Prod. 2016, 87, 177–181. Arbenz, A.; Frache, A.; Cuttica, F.; Avérous, L. Advanced biobased and rigid foams, based on urethane-modified isocyanurate from oxypropylated gambier tannin polyol. Polym. Degrad. Stab. Merle, J.; Birot, M.; Deleuze, H.; Mitterer, C.; Carre, H.; Bouhtoury, F. C.-E. New biobased foams from wood byproducts. Mater. Des. 2016, 91, 186–192. 23 ACS Paragon Plus Environment


1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

(21)

(22) (23) (24) (25) (26) (27)

(28) (29)

(30) (31)

(32)

(33)

(34) (35) (36) (37) (38)

(39)

Page 24 of 25

Polgar, L. M.; van Duin, M.; Broekhuis, A. A.; Picchioni, F. Use of Diels–Alder Chemistry for Thermoreversible Cross-Linking of Rubbers: The Next Step toward Recycling of Rubber Products? Macromolecules 2015, 48 (19), 7096–7105. Hager, M. D.; Bode, S.; Weber, C.; Schubert, U. S. Shape memory polymers: Past, present and future developments. Prog. Polym. Sci. 2015, 49–50, 3–33. Rivero, G.; Nguyen, L.-T. T.; Hillewaere, X. K. D.; Du Prez, F. E. One-Pot ThermoRemendable Shape Memory Polyurethanes. Macromolecules 2014, 47 (6), 2010–2018. Hillewaere, X. K. D.; Du Prez, F. E. Fifteen chemistries for autonomous external self-healing polymers and composites. Prog. Polym. Sci. 2015, 49–50, 121–153. Liu, Y.-L.; Chuo, T.-W. Self-healing polymers based on thermally reversible Diels–Alder chemistry. Polym. Chem. 2013, 4 (7), 2194–2205. Tillet, G.; Boutevin, B.; Ameduri, B. Chemical reactions of polymer crosslinking and postcrosslinking at room and medium temperature. Prog. Polym. Sci. 2011, 36 (2), 191–217. Froidevaux, V.; Negrell, C.; Laborbe, E.; Auvergne, R.; Boutevin, B. Thermosetting material by a thermo responsive cross-linking using retroDiels-Alder and, in situ, Thia-Michael reactions. Eur. Polym. J. 2015, 69, 510–522. Sanyal, A. Diels–Alder Cycloaddition-Cycloreversion: A Powerful Combo in Materials Design. Macromol. Chem. Phys. 2010, 211 (13), 1417–1425. Gevrek, T. N.; Arslan, M.; Sanyal, A. Design and Synthesis of Maleimide Group Containing Polymeric Materials via the Diels-Alder/Retro Diels-Alder Strategy. In Functional Polymers by Post-Polymerization Modification; Theato, P., Klok, H.-A., Eds.; Wiley-VCH Verlag GmbH & Co. KGaA, 2012; pp 119–151. Gandini, A. The furan/maleimide Diels–Alder reaction: A versatile click–unclick tool in macromolecular synthesis. Prog. Polym. Sci. 2013, 38 (1), 1–29. Granata, A.; Argyropoulos, D. S. 2-Chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane, a Reagent for the Accurate Determination of the Uncondensed and Condensed Phenolic Moieties in Lignins. J. Agric. Food Chem. 1995, 43 (6), 1538–1544. Melone, F.; Saladino, R.; Lange, H.; Crestini, C. Tannin Structural Elucidation and Quantitative 31P NMR Analysis. 1. Model Compounds. J. Agric. Food Chem. 2013, 61 (39), 9307–9315. Melone, F.; Saladino, R.; Lange, H.; Crestini, C. Tannin Structural Elucidation and Quantitative 31P NMR Analysis. 2. Hydrolyzable Tannins and Proanthocyanidins. J. Agric. Food Chem. 2013, 61 (39), 9316–9324. Duval, A.; Lange, H.; Lawoko, M.; Crestini, C. Reversible crosslinking of lignin via the furan– maleimide Diels–Alder reaction. Green Chem. 2015, 17 (11), 4991–5000. Duval, A.; Avérous, L. Characterization and Physicochemical Properties of Condensed Tannins from Acacia catechu. J. Agric. Food Chem. 2016, 64 (8), 1751–1760. Madhavaram, H.; Idriss, H. Acetaldehyde reactions over the uranium oxide system. J. Catal. 2004, 224 (2), 358–369. Aguiar, E. C.; da Silva, J. B. P.; Ramos, M. N. A theoretical study of the vibrational spectrum of maleimide. J. Mol. Struct. 2011, 993 (1–3), 431–434. Dolci, E.; Michaud, G.; Simon, F.; Boutevin, B.; Fouquay, S.; Caillol, S. Remendable thermosetting polymers for isocyanate-free adhesives: a preliminary study. Polym. Chem. 2015, 6 (45), 7851–7861. Froidevaux, V.; Borne, M.; Laborbe, E.; Auvergne, R.; Gandini, A.; Boutevin, B. Study of the Diels–Alder and retro-Diels–Alder reaction between furan derivatives and maleimide for the creation of new materials. RSC Adv. 2015, 5 (47), 37742–37754.


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For Table of Contents use only Biobased and aromatic reversible thermoset networks from condensed tannins via the Diels-Alder reaction Antoine Duval, Guillaume Couture, Sylvain Caillol, Luc Avérous

Biobased aromatic thermoreversible polymer networks were prepared from the wood polyphenol tannins modified with furan groups and bismaleimide linkers.


Biobased and Aromatic Reversible Thermoset Networks from

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