Biomimetic Domain-Active Electrospun Scaffolds Facilitating Bone

Nov 27, 2017 - To improve bone regeneration in oral microenvironment, we generated a novel biodegradable, antibacterial and osteoconductive electrospu...
0 downloads 9 Views 2MB Size
Subscriber access provided by UNIVERSITY OF CONNECTICUT

Article

Biomimetic Domain-Active Electrospun Scaffolds Facilitating Bone Regeneration Synergistically with Antibacterial Efficacy for Bone Defects YunZhu Qian, Xuefeng Zhou, Hong Sun, Jianxin Yang, Yi Chen, Chao Li, Hongjin Wang, Tong Xing, FeiMin Zhang, and Ning Gu ACS Appl. Mater. Interfaces, Just Accepted Manuscript • DOI: 10.1021/acsami.7b14524 • Publication Date (Web): 27 Nov 2017 Downloaded from http://pubs.acs.org on November 28, 2017

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

ACS Applied Materials & Interfaces is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 34 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

ACS Applied Materials & Interfaces

Biomimetic Domain-Active Electrospun Scaffolds Facilitating Bone Regeneration Synergistically with Antibacterial Efficacy for Bone Defects

Yunzhu Qian,†,⊥ Xuefeng Zhou, ‡,⊥ Hong Sun,§ Jianxin Yang,† Yi Chen,‡ Chao Li,§ Hongjin Wang,§ Tong Xing,§ Feimin Zhang,*,∥ and Ning Gu*,‡ † Center of Stomatology, the Second Affiliated Hospital of Soochow University. Suzhou 215004, PR China ‡ State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China ∥ Xi’an Jiaotong University Suzhou Research Institute, Suzhou 215123, PR China

† Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, PR China

1

ACS Paragon Plus Environment

ACS Applied Materials & Interfaces 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Page 2 of 34

Abstract To improve bone regeneration in oral microenvironment, we generated a novel biodegradable, antibacterial and osteoconductive electrospun PLGA/PCL membrane as an ideal osteogenic scaffold. The novel 3-layer membranes were structured with serial layers of electrospun chlorhexidine-doped-PLGA/PCL

(PPC),

PLGA/PCL

(PP),

and

β-tricalcium

phosphate-doped-PLGA/PCL (PPβ). To characterize osteoconductive properties of these membranes, MC3T3-E1 (MC) cultures were seeded onto the membranes for 14 days for evaluation of cell proliferation, morphology and gene/protein expression. In addition, MC cells were cultured onto different surfaces of the 3-layer membranes, PPC layer facing MC cells (PPβ-PP-PPC) and PPβ layer facing MC cells (PPC-PP-PPβ) to evaluate surface-material effects. Membrane properties and structures were evaluated. Antibacterial properties against S. mutans and S. aureus were determined. Scanning electron microscope demonstrated smaller interfiber spaces of PPC and PPβ-PP-PPC compared to PPβ, PPC-PP-PPβ and PP. PPC and PPβ-PP-PPC exhibited hydrophilic property. The 3-layer membranes (PPC-PP-PPβ and PPβ-PP-PPC) demonstrated significantly higher Young’s modulus (94.99±4.03MPa and 92.88±4.03MPa) compared to PP (48.76±18.15MPa) or PPC (7.92±3.97MPa) (P0.05). Higher expression of Integrins were detected at 12h of cultures on PPC-PP-PPβ compared to the controls. Promoted osteoconductive effects of PPC-PP-PPβ was revealed by ALP assays and Western blot compared with the controls at 7 and 14 days. PPC, PPC-PP-PPβ and PPβ-PP-PPC exhibited significantly wider antibacterial zone against the tested bacteria compared to PP and PPβ (P