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Biomimetic Domain-Active Electrospun Scaffolds Facilitating Bone Regeneration Synergistically with Antibacterial Efficacy for Bone Defects YunZhu Qian, Xuefeng Zhou, Hong Sun, Jianxin Yang, Yi Chen, Chao Li, Hongjin Wang, Tong Xing, FeiMin Zhang, and Ning Gu ACS Appl. Mater. Interfaces, Just Accepted Manuscript • DOI: 10.1021/acsami.7b14524 • Publication Date (Web): 27 Nov 2017 Downloaded from http://pubs.acs.org on November 28, 2017
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ACS Applied Materials & Interfaces
Biomimetic Domain-Active Electrospun Scaffolds Facilitating Bone Regeneration Synergistically with Antibacterial Efficacy for Bone Defects
Yunzhu Qian,†,⊥ Xuefeng Zhou, ‡,⊥ Hong Sun,§ Jianxin Yang,† Yi Chen,‡ Chao Li,§ Hongjin Wang,§ Tong Xing,§ Feimin Zhang,*,∥ and Ning Gu*,‡ † Center of Stomatology, the Second Affiliated Hospital of Soochow University. Suzhou 215004, PR China ‡ State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China ∥ Xi’an Jiaotong University Suzhou Research Institute, Suzhou 215123, PR China
† Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210029, PR China
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Abstract To improve bone regeneration in oral microenvironment, we generated a novel biodegradable, antibacterial and osteoconductive electrospun PLGA/PCL membrane as an ideal osteogenic scaffold. The novel 3-layer membranes were structured with serial layers of electrospun chlorhexidine-doped-PLGA/PCL
(PPC),
PLGA/PCL
(PP),
and
β-tricalcium
phosphate-doped-PLGA/PCL (PPβ). To characterize osteoconductive properties of these membranes, MC3T3-E1 (MC) cultures were seeded onto the membranes for 14 days for evaluation of cell proliferation, morphology and gene/protein expression. In addition, MC cells were cultured onto different surfaces of the 3-layer membranes, PPC layer facing MC cells (PPβ-PP-PPC) and PPβ layer facing MC cells (PPC-PP-PPβ) to evaluate surface-material effects. Membrane properties and structures were evaluated. Antibacterial properties against S. mutans and S. aureus were determined. Scanning electron microscope demonstrated smaller interfiber spaces of PPC and PPβ-PP-PPC compared to PPβ, PPC-PP-PPβ and PP. PPC and PPβ-PP-PPC exhibited hydrophilic property. The 3-layer membranes (PPC-PP-PPβ and PPβ-PP-PPC) demonstrated significantly higher Young’s modulus (94.99±4.03MPa and 92.88±4.03MPa) compared to PP (48.76±18.15MPa) or PPC (7.92±3.97MPa) (P0.05). Higher expression of Integrins were detected at 12h of cultures on PPC-PP-PPβ compared to the controls. Promoted osteoconductive effects of PPC-PP-PPβ was revealed by ALP assays and Western blot compared with the controls at 7 and 14 days. PPC, PPC-PP-PPβ and PPβ-PP-PPC exhibited significantly wider antibacterial zone against the tested bacteria compared to PP and PPβ (P
