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BIOTECHNOLOGY PROGRESS Biotechnology Progress (ISSN 8756-7938) is copublished bimonthly by the American Chemical Society, 1155 16th St., N.W., Washington, DC 20036, and the American Institute of Chemical Engineers, 345 East 47th St., New York, NY 10017. Second-class postage paid at New York, NY, and additional mailing offices. POSTMASTER: Send address changes to Biotechnology Progress, Member & S u b s c r i b e r S e r v i c e s , P . O . Box 3337, Columbus, OH 43210 (614) 447-3776; (800) 333-9511. Canadian GST Reg. No. R127571347. Printed in the USA. Published with the cooperation of the Society for Industrial Microbiology, 3929 Old Lee Highway, Fairfax, VA 22030; (703) 691-3357. MANAGEMENT BOARD American Chemical Society Robert H. Marks Charles R. Bertsch American Institute of Chemical Engineers Stephen R. Smith Mark D. Rosenzweig Journals Department American Chemical Society 2540 Olentangy River Road P.O. Box 3330 Columbus, OH 43210 (614) 447-3600, Ext. 3171 TELEX 6842086 FAX (614) 447-3745 Manager: Mary E. Scanlan Journals Editing Manager: Anne C. O'Melia Assistant Editor: Alena R. Miller-Roush Publications Directorate American Institute of Chemical Engineers 345 East 47th Street New York, NY 10017 (212) 705-7338 FAX (212) 752-3294 Managing Editor: Maura Mullen Advertising Office: Centcom, Ltd., 1599 Post Road East, P.O. Box 231, Westport, CT 06881

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BIOTECHNOLOGY PROGRESS®

VOLUME 11, NUMBER 3

May/June 1995

Copyright 1995 by the American Chemical Society and the American Institute of Chemical Engineers

BIPRET 11(3) 235-356 (1995)

ISSN 8756-7938

EDITOR JEROME S. SCHULTZ C e n t e r for Biotechnology a n d Bioengineering University of P i t t s b u r g h P i t t s b u r g h , P e n n s y l v a n i a 15219 Phone: (412) 383-9712 Fax: (412) 383-9710

ASSOCIATE EDITOR, REVIEWS MICHAEL C. FLICKINGER I n s t i t u t e for Advanced Studies in Biological Process Technology University of M i n n e s o t a St. P a u l , M i n n e s o t a 55108 Phone: (612) 624-9706 Fax: (612) 625-1700

REVIEW

235 Biosorption of Heavy Metals B. V o l e s k y * a n d Z. R. H o l a n

ADVISORY BOARD Fredric Bader Auto Immune James Bailey ΕΤΗ, Zurich Kenneth Bischoff University of Delaware Bruce Dale Texas A&M University J o h n Gerlt University of Maryland Juan Hong University of California—Irvine J e n n i f e r C. H u n t e r - C e v e r a Society for I n d u s t r i a l Microbiology Rakesh Jain Harvard Medical School John Jost Genentech, Inc. Alexander Klibanov Massachusetts Institute of Technology Michael Ladisch Purdue University Douglas Lauffenburger University of Illinois, Urbana-Champaign Daryl Lund Rutgers University Janice Phillips Lehigh University Sharon Shoemaker University of California—Davis Michael Shuler Cornell University Gregory Stephanopoulos Massachusetts Institute of Technology JoAnn Stubbe Massachusetts Institute of Technology James Swartz Genentech, Inc. Elizabeth Theil North Carolina State University

K. Venkat Phyton Catalytic, Inc Henry Wang University of Michigan Howard Weetall National Institute of Science and Technology

TOPICAL PAPER 251 Microbial Polysaccharides of Applied Interest: Ongoing Research Activities in Europe Vittorio Crescenzi BIOSEPARATIONS AND DOWNSTREAM PROCESSING 260 Extraction of Charged Fusion Proteins in Reversed Micelles: Comparison between Different Surfactant Systems Craig E. Forney and Charles E. Glatz* 265 Purification by Immobilized Metal Affinity Chromatography of Human Atrial Natriuretic Peptide Expressed in a Novel Thioredoxin Fusion Protein David L. Wilkinson, Nien-Tung Ma, Chris Haught, and Roger G. Harrison* 270 Surfactant-Coated Lipase Suitable for the Enzymatic Resolution of Menthol as a Biocatalyst in Organic Media Noriho Kamiya, Masahiro Goto,* and Fumiyuki Nakashio APPLIED CELLULAR PHYSIOLOGY AND METABOLIC ENGINEERING 276 Investigation of the Ethanol Tolerance of Clostridium thermosaccharolyticum in Continuous Culture Sunitha Baskaran, Hyung-Jun Ahn, and Lee R. Lynd* 282 Specificity of a Lipase in Ester Synthesis: Effect of Alcohol Neena N. Gandhi, Sudhirprakash B. Sawant,* and Jyeshtharaj B. Joshi 288 Fnr, a Global Transcriptional Regulator of Escherichia coli, Activates the Vitreoscilla Hemoglobin (VHb) Promoter and Intracellular VHb Expression Increases Cytochrome d Promoter Activity Philip S. Tsai, Pauli T. Kallio, and James E. Bailey*