ARTICLE pubs.acs.org/est
Blood Plasma Sample Preparation Method for the Assessment of Thyroid Hormone-Disrupting Potency in Effect-Directed Analysis Eszter Simon,† Jenny Bytingsvik,‡ Willem Jonker,† Pim E. G. Leonards,† Jacob de Boer,† Bjørn M. Jenssen,‡ Elisabeth Lie,§ Jon Aars,|| Timo Hamers,† and Marja H. Lamoree*,† †
Institute for Environmental Studies (IVM), VU University Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands Department of Biology, Norwegian University of Science and Technology (NTNU), Høgskoleringen 5, NO-7491 Trondheim, Norway § Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science (NVH), NO-0033 Oslo, Norway Norwegian Polar Institute (NPI), Fram Centre, NO-9296 Tromsø, Norway
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bS Supporting Information ABSTRACT: A sample preparation method combining solid-phase extraction (SPE) and liquidliquid extraction (LLE) was developed to be used in Effect-Directed Analysis (EDA) of blood plasma. Until now such a method was not available. It can be used for extraction of a broad range of thyroid hormone (TH)-disruptors from plasma with high recoveries. Validation of the method using spiked cow plasma showed good recoveries for hydroxylated polybrominated diphenyl ethers (OH-PBDEs; 93.8 ( 19.5%), hydroxylated polychlorinated biphenyls (OH-PCBs; 93.8 ( 15.5%), other halogenated phenols (OHPs; 107 ( 8.1%), and for short-chain (
