books and software
A New Gateway for LC/MS
LC/MS Applications in Drug Development Mike S. Lee John Wiley & Sons, 2002, 235 pp, $89.95
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his technical review is organized into nine chapters, a glossary, and a reference section. Chapters 1–5 and 7–9 constitute approximately one-third of the text. These chapters introduce the subject of drug development, discuss broad strategies for the application of LC/MS to drug development, and reflect on prospects for future LC/MS applications. Some of these chapters focus on organizational development. Chapter 6 comprises nearly half of the text and gives a comprehensive and up-to-date overview of LC/MS applications in drug development. References from as recently as 2001 are cited. Chapters 2 and 4 are good starting points for newcomers to LC/MS in drug development. The four stages of drug development—drug discovery, preclinical development, clinical development, and manufacturing—are introduced in chapter 2. This overview brings together the corporate objectives, general analytical needs, and potential LC/ MS utilities for each stage. Chapter 4 introduces LC/MS development. The chapter 3 focus on accelerated drug development and the chapter 5 discussion on analysis strategies take the reader to the next introductory level. The author defines nine approaches that he believes are relevant to the application of LC/MS in drug development. These methods apply to quantitative and qualitative analyses, pharmaceutical properties, and productivity issues. Chapter 6 is organized into sections based on the four stages of drug develop-
ment outlined in chapter 2. Each section includes a discussion of analysis requirements, analysis perspectives, and LC/MS contributions, as well as an overview. The author then targets several representative application areas and examines specific examples in detail. For example, in the drug discovery section, the discussions of proteomics in support of target identification and combinatorial chemistry in support of lead optimization provide good overviews of these fields. The examples of LC/MS applications in the areas of membrane permeability, drug–drug interactions, and metabolic stability will be of particular interest to those working in the later phases of drug discovery. In the preclinical development section, the author gives appropriate attention to the metabolite, impurity, and degradant identification activities associated with this stage. However, the absence of any discussion on quantitative LC/MS support for preclinical absorption, distribution, metabolism, and elimination studies is surprising, given their scope and importance. One focus in the clinical development section is the growing importance of rap-
id, high-capacity, automated sample preparation procedures supporting clinical quantitative LC/MS. The author discusses several off- and on-line extraction strategies. The time and cost savings associated with an off-line, 96-well disk plate, solid-phase extraction procedure are examined in detail. The popular on-line extraction strategies involving turbulentflow chromatography and restrictedaccess media are also referenced. Important LC/MS activities supporting production, quality control, and patent protection are well presented in the manufacturing section of chapter 6. This book is a comprehensive primer and will appeal to laboratory scientists and instructors in the pharmaceutical field. Analytical managers will especially appreciate the elements of organizational development woven throughout the text. Anyone involved in LC/MS will find this book a great gateway to many references on this field. Reviewed by Robert L. St. Claire III of Triangle Pharmaceuticals, Inc.
Books Received b b Single Molecule Detection in Solution Edited by Christoph Zander, Jörg Enderlein, and Richard Keller John Wiley & Sons, 2002, 365 pp, $145 Single-molecule detection and the field of nanoscience have expanded during the past 10 years. This book covers the gambit, from the basics to the latest applications and results. Surface-enhanced Raman scattering, surface detection with a confocal laser scanning microscope, and the spectroscopy of individual photosynthetic pigment proteins are some of the topics covered in detail.
b The Raman Effect Derek A. Long John Wiley & Sons, 2002, 585 pp, $160 This edition makes a broad sweep over the Raman spectroscopy field. The first part has 10 chapters covering
subjects such as vibrational Raman scattering, the quantum mechanical theory of Rayleigh and Raman scattering, and the survey of light-scattering phenomena. The second part has 21 chapters and covers topics such as direction cosines, vectors, tensors, and sources of electromagnetic radiation.
b Modern Fourier: Transform Infrared Spectroscopy A. A. Christy, Y. Ozaki, and V. G. Gregoriou Elsevier, 2001, 351 pp, $169 This book is part of the Comprehensive Analytical Chemistry series and covers not only the fundamental and applied aspects of infrared spectroscopy but also recent vibrational and hyphenated chromatographic techniques. Other applications discussed include polymeric and biological materials, chemometric interpretation, and data analysis.
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