Books: Conceptual and practical information - Analytical Chemistry

Books: Conceptual and practical information. A review of Enzyme Immunoassays: From Concept to Product Development. Theodore K. Christopoulos. Anal...
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When there's only a trace Conceptual and practical information

Trace Analysis: A Structured Approach to Obtaining Reliable Results E. Prichard, G. M. MacKay, and J. Points, Eds. Royal Society of Chemistry c/o American Chemical Society 1155 16th St., N.W. Washington, D.C. 20036 1996, 404 pp.. $125

This book, which was prepared as part of the Valid Analytical Measurement project in the United Kingdom, begins with an interesting and detailed introduction to achieving valid trace analysis and then addresses critically important aspects of sample handling—pretreatment and storage. Although sampling itself is not covered in detail, the reader is referred regularly to relevant literature. Chapters 3 and 4 deal with sample preparation for determining inorganic analytes and emphasize valid analytical determination. In this respect, the book is an improvement many analytical chemistry textbooks. Chapters 5 and 6 are devoted to the problems of organic analyses. Sample preparation methods are followed by measurement methods, emphasizing GC, HPLC, TLC, MS, and isotope dilution MS. The authors give overviews and practical advice on the methods and discuss critical aspects and associated uncertainties of the analytical measurement. These two chapters constitute the bulk of the text and are helpful to the practicing analytical 184 A

chemist. They are filled with commonsense comments from experienced analytical chemists, and they give a refreshingly pragmatic approach to the problems described. The chapter on the analysis of speciated elements starts off boldly but falls flat amidst the minutiae of certain instrumental methods and wanders off the subject. From this point, the text shifts from a structured approach for obtaining reliable results to a description of techniques suitable for both inorganic and organic analytes, with references to methods of ensuring the quality of the instrumentation and maintaining realistic response. It is rather a pity that the authors took this Enzyme Immunoassays: route because some of the comments in From Concept to Product earlier chapters would have been approDevelopment priate and useful in the sections on elecS. S. Deshpande trochemical techniques. Chapman & Hall 115 Fifth Ave. I was disappointed with the chapter on New York, NY 10003 signal processing and reporting of results (Chapter 9). I did notfindit to be a signifi- 1996, 464 pp.. $110 cant or well-written contribution to the book. The section on signal integrity left Thefirstseven chapters cover general me wondering whether the authors really aspects of immunoassay reagents and sepknew their subject. The section on data aration systems. Following an introduchandling was a precis of basic statistical tory chapter, Chapters 2 and 3 discuss methods and was quite well done. Howbasic concepts of antibodies. Chapter 2 ever, it might have been advisable to treat describes the biology of the immune syssuch an important topic in a separate text tem; antibody classification, structure, and or to treat it with a depth that reflected its function; and, briefly, other binding proimportance in the reporting of trace anateins used in various immunoassay configlytical data. The only significant error that urations. Chapter 3 discusses the fundaI found in the book was a rather suspect mental aspects of antigen-antibody interequation in the data-handling section action and elaborates on the optimization of the competitive-type immunoassay. Much of this book is useful to the analytical chemist in carrying out trace analyChapters 4 and 5 deal with issues sursis. It is practical, helpful, modern, and, in rounding the immunogen used in the asmany respects, ageless. There is room for say. Chapter 4 focuses on conjugation improvement in certain areas, and I think strategies used in the preparation of the that the next generations of the text will immunogen or the tracer. Extensive lists be even more valuable than this one. of homobifunctional and heterobifuncReviewed by J. F. Alder, University of tional crosslinkers are provided. Although Manchester Instititu of Science and Tech- conjugation protocols are not given, several references are cited. This chapter nology yU.K.)

Analytical Chemistry News & Features, March 1, 1997

would have been improved by including figures that tllustrate imporrant and frequently used conjugation reactions. Chapter 5 focuses on the production of polyclonal and monoclonal antibodies, covering the preparation of the immunogen (with useful examples), immunization, antibody screening, antibody purification stabilization, and storage. Chapter 6 discusses the various enzymes used in immunoassays. It provides useful information on the physicochemical properties of each enzyme, the reaction mechanism, the substrates, and conjugation methods for the particular enzyme. Amplification systems involving enzyme-antienzyme complexes, coupled enzyme cascades, and the biotin-avidin interaction are also described. Separation systems and various solid phases are adequately covered in chapter 7. The last five chapters cover the practical rather than the conceptual. In chapter 8, the author classifies immunoassays and describes several commercial immunoassay systems. The steps involved in assay development, assay evaluation, and quality assurance are extensively covered in Chapter 9. Moreover, it describes the principles and procedures used in assay validation for clinical, environmental, and food diagnostics. Chapter 10 gives an excellent and detailed description of the formulation, preparation, and shelf-life evaluation of liquid and solid reagents used in immunodiagnostic kits. The various methods used for graphical presentation and fitting of immunoassay data Off**

books and will be most useful to those inChiral Separations: Applicavolved in the development of diagnostic kits tions and Technology based on enzyme immunoassays. Satinder Ahuja, Ed. Reviewed bb Theodore K. Christopoulos, American Chemical Society University of Windsor, Ontario, Canada 1155 16th St., N.W. Washington, DC 20036 1997, 349 pp., $99.95 BOOKS RECEIVED

Principles of Environmental Sampling 2nd e d . Lawrence H. Keith, Ed. American Chemical Society 1155 16th St., N.W. Washington, DC 20036 1996, 848 pp., $99.95

This edition, with 24 new chapters and nearly twice the material, updates the original to reflect changes in environmental regulations. It covers sampling and sample preservation in all applicable matrices. The theory of quality control and assurance is discussed, as well as the special quality concerns of particular matrices. An extensive subject index is included. Environmental Immunochemical Methods: Perspectives and Applications Jeanette M. Van Emon, Clare L. Gerlach, and Jeffre C. Johnson, Eds. American Chemical Society 1155 16th St.. N.W. Washington, DC 20036 1996, 342 pp., $109.95

This book draws information from the deregulatory, academic, and industrial comscribed in chapter 11 The final chapter munities about the acceptance of, adfocuses on the design of good laboratory and manufacturing practices Useful infor- vances in, and commercialization of immunochemical methods of environmental mation about start-up companies is proanalysis. The 27 contributed chapters are wded at the end of this chapter based on the National Immunochemistry The material is well presented, and each Summit IV. Author, affiliation, and subject chapter is followed by a list of key references. The last five chapters make this con- indexes are included. tribution unique among immunoassay

Despite the title, this book deals with the preparation and isolation, as well as the separation, of chiral molecules. It includes pharmaceutical, agricultural, biological, and synthetic organic applications. A subject index is included. Spot Test Analysis 2nd ed. Ervin Jungreis John Wiley & Sons 605 Third Ave. New York, NY 10158 1997, 377pp,, $69.95

This book explains the contemporary uses of spot tests and describes techniques for clinical, geochemical, environmental, and forensic applications. It includes immunoassays for cholesterol, HIV, drugs, and explosives. A subject index is included. Total-Reflection X-Ray Fluorescence Analysis Reinhold Klockenkämper John Wiley & Sons 605 Third Ave. New York, NY 10158 1997,245 pp., $69.95

Following an introduction to the fundamental principles of X-ray fluorescence and total-reflection XRF, this book describes the instrumentation used inTRXRF, including sources, beam-adapting units, and detectors. Clinical, medical, environmental, and art historical applications are discussed. A subject index is included.

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