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CHROMATOGRAPHY CatalogNo.45
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cerning such topics as loading capacity, numbers of septa required for stability, and sample dispersion. Essentially nothing is said about other rotor configurations. The chapter on analytical liquid centrifuges is in three parts: a standard but limited description of the use of the analytical ultracentrifuge; a lengthy discussion of an obscure use of the preparative centrifuge that attempts to measure the interaction energies of antigen-antibody complexes immobilized on latex beads; and another lengthy discussion, this on the centrifugal clinical analyzer. This latter device does not characterize particles on the basis of their sedimentation properties, but uses centrifugal force to mix and transfer reagent and analyte solutions. Its inclusion in a book titled "Separations by Centrifugal Phenomena" is perhaps, therefore, moot. A chapter titled "Liquid Centrifugation in Practice" follows. This is devoted almost exclusively to separations in density gradients from the macromolecular to the organelle level. The contents of this book reflect to a large extent the inventive genius of Norman Anderson, who both conceived and brought into being zonal centrifuges and the clinical analyzer. This is quite proper, for Anderson's contributions to centrifugal technology are profound. The contents also reflect the author's limited experience in centrifugation in general. At the least, this results in confusing usage. For example, "sedimentation equilibrium" is interchanged freely with "isopycnic banding," and while systems that are banded isopycnically are at sedimentation equilibrium, the latter term is usually reserved for molecular weight determinations in the analytical ultracentrifuge. Hsu also identifies a pseudoequilibrium state prior to the isopycnic banding of an initially layered zone as the "approach to equilibrium method"—a term used, again in analytical ultracentrifuge methodology, for the Archibald method. There are numerous omissions (the planetary centrifuge; tjie centrifugal elutriator; the Yphantis, or meniscus depletion method for molecular weight determinations; the centrifugal blood cell separator; laser, Vidicon and other optical systems for the analytical ultracentrifuge; and the study of interacting systems in the analytical ultracentrifuge); and some dubious inclusions, which have already been referred to. Although the book claims to create a "coherent framework of transport phenomena" we are treated to at least three different derivations of the basic sedimentation equations and no fewer than 16 pages of symbols!
734 A · ANALYTICAL CHEMISTRY, VOL. 54, NO. 6, MAY 1982
The most disturbing feature of the book is that it produces a pervasive feeling of déjà vu, particularly in the nontheoretical parts, although it is present in the theoretical sections to a lesser extent. This is due to freely using, verbatim, paragraphs and sections of other texts—Fujita's "Foundations of Ultracentrifugal Analysis"; "Methods of Biochemical Analysis, volume 23"; "Chemical Separations and Measurements" by Peters, Hayes, and Hieftje; and "Centrifugal Separations in Molecular and Cell Biology" by Birnie and Rockwood to name a few. This book cannot be recommended. It appears to be primarily a forum for the author's own papers in theoretical aspects of centrifugation set in material abstracted from other texts. Those interested in centrifugal analysis and separations will find more complete and considered treatments of these subjects in the volumes listed above (with the exception of Peters, Hayes, and Hieftje) together with "Centrifugation in Biology and Medical Science" by Sheeler.
Books Received Affinity Chromatography and Related Techniques. T. C. J. Gribnau, J. Visser, R. J. F. Nivard, Eds. xviii + 584 pp. Elsevier North-Holland, Inc., 52 Vanderbilt Ave., New York, N.Y. 10017. 1982. $83 Ion Exchange and Solvent Extraction. Vol. 8, Jacob Marinsky, Yizhak Marcus, Eds. xi + 438 pp. Marcel Dekker, Inc., 270 Madison Ave., New York, N.Y. 10016. 1981. $55 Calculator Programming for Chemistry and the Life Sciences. Frank Clark, vii + 226 pp. Academic Press, Inc., 111 Fifth Ave., New York, N.Y. 10003. 1981. $24.50 Comprehensive Treatise of Electrochemistry. Vol. 4. J. O'M. Bockris et al., Eds. xxii + 563 pp. Plenum Press, 233 Spring St., New York, N.Y. 10013. 1981. $55 NMR in Molecular Biology. Oleg Jardetzky, G.C.K. Roberts, xiii + 681 pp. Academic Press, 111 Fifth Ave., New York, N.Y. 10003. 1981. $59 Nuclear and Chemical Dating Techniques: Interpreting the Environmental Record. Lloyd Currie, Ed. xi 4- 516 pp. American Chemical Society, 1155 Sixteenth St., N.W., Washington, D.C. 20036. 1982. $48.95
