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Bioactive Constituents, Metabolites, and Functions
Broccoli sprout extract Alleviates Alcohol-Induced Oxidative Stress and Endoplasmic Reticulum Stress in C57BL/6 Mice Peng Lei, Wei Zhao, Bo Pang, Xiuli Yang, Bao-Long Li, Minghua Ren, and Yu-Juan Shan J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b01653 • Publication Date (Web): 06 May 2018 Downloaded from http://pubs.acs.org on May 6, 2018
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Journal of Agricultural and Food Chemistry
Broccoli sprout extract Alleviates Alcohol-Induced Oxidative Stress and Endoplasmic Reticulum Stress in C57BL/6 Mice Peng Leia#, Wei Zhaoa#, Bo Panga, Xiuli Yanga, Bao-Long Lib,c# Minghua Rend, Yu-Juan Shana* a
Department of Food Science and Engineering, Harbin Institute of Technology, No. 73
Huanghe Road, Harbin 150090, China b
Center for Drug Safety Evaluation, Heilongjiang University of Chinese Medicine, No. 24
Heping Road, Harbin 150040, China c
Post-Doctoral Research Center in Pharmacy, Harbin Medical University, No.94 Baojian
Road, Harbin, 150086, China d
Department of Urinary Surgery, the First Affiliated Hospital of Harbin Medical University,
No.23 Youzheng Street, Harbin 150001, China E-mails:
[email protected] (L.P);
[email protected] (Z.W);
[email protected] (L.-B.L);
[email protected] (P.
B);
[email protected] (Y.-X.L.);
[email protected] (R.-M.H.) * Corresponding author; E-Mails:
[email protected] (Y.-J.S.), Tel: +86-451-8628-2021 (Y.-J.S.); Fax: +86-451-8628-2906 (Y.-J.S.). #
Contributed equally to this work.
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Journal of Agricultural and Food Chemistry
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Abstract
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The potential efficacy of sulforaphane in protecting alcohol-induced hepatic injury in vivo
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and its underlying mechanism was investigated. Male C57BL/6 mice were orally
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administrated with broccoli sprout extract (BSE) containing sulforaphane (7.6, 25.2, 50.4
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mg/kg.bw) once a day for 14 days. At the 13th day, mice were challenged with alcohol
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(5g/kg.bw) every 12 h for 3 times, which increased malondialdehyde (MDA) levels
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(4.44±1.24 nmol/mg prot, p
