Broccoli Sprout Extract Alleviates Alcohol-Induced Oxidative Stress

May 6, 2018 - (1−3) Alcohol administration causes accumulation of reactive oxygen species .... The slides were subsequently incubated with murine mo...
2 downloads 0 Views 4MB Size
Subscriber access provided by UNIVERSITY OF THE SUNSHINE COAST

Bioactive Constituents, Metabolites, and Functions

Broccoli sprout extract Alleviates Alcohol-Induced Oxidative Stress and Endoplasmic Reticulum Stress in C57BL/6 Mice Peng Lei, Wei Zhao, Bo Pang, Xiuli Yang, Bao-Long Li, Minghua Ren, and Yu-Juan Shan J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b01653 • Publication Date (Web): 06 May 2018 Downloaded from http://pubs.acs.org on May 6, 2018

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 29

Journal of Agricultural and Food Chemistry

Broccoli sprout extract Alleviates Alcohol-Induced Oxidative Stress and Endoplasmic Reticulum Stress in C57BL/6 Mice Peng Leia#, Wei Zhaoa#, Bo Panga, Xiuli Yanga, Bao-Long Lib,c# Minghua Rend, Yu-Juan Shana* a

Department of Food Science and Engineering, Harbin Institute of Technology, No. 73

Huanghe Road, Harbin 150090, China b

Center for Drug Safety Evaluation, Heilongjiang University of Chinese Medicine, No. 24

Heping Road, Harbin 150040, China c

Post-Doctoral Research Center in Pharmacy, Harbin Medical University, No.94 Baojian

Road, Harbin, 150086, China d

Department of Urinary Surgery, the First Affiliated Hospital of Harbin Medical University,

No.23 Youzheng Street, Harbin 150001, China E-mails: lp995842062@163.com (L.P); zhaowei78@hit.edu.cn (Z.W); lbl73@163.com (L.-B.L);

13633647758@163.com

(P.

B);

yangxiuli777@163.com

(Y.-X.L.);

renminghua@163.com (R.-M.H.) * Corresponding author; E-Mails: shanyujuan@hit.edu.cn (Y.-J.S.), Tel: +86-451-8628-2021 (Y.-J.S.); Fax: +86-451-8628-2906 (Y.-J.S.). #

Contributed equally to this work.

1

ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry

Page 2 of 29

1

Abstract

2

The potential efficacy of sulforaphane in protecting alcohol-induced hepatic injury in vivo

3

and its underlying mechanism was investigated. Male C57BL/6 mice were orally

4

administrated with broccoli sprout extract (BSE) containing sulforaphane (7.6, 25.2, 50.4

5

mg/kg.bw) once a day for 14 days. At the 13th day, mice were challenged with alcohol

6

(5g/kg.bw) every 12 h for 3 times, which increased malondialdehyde (MDA) levels

7

(4.44±1.24 nmol/mg prot, p