Chem. Res. Toxicol. 1997, 10, 1387-1396
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(Carboxyalkyl)pyrroles in Human Plasma and Oxidized Low-Density Lipoproteins Kamaljit Kaur,† Robert G. Salomon,*,† June O’Neil,‡ and Henry F. Hoff‡ Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106-7078, and Department of Cell Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195 Received June 30, 1997X
Free-radical oxidation of human plasma low-density lipoprotein (LDL) produces (carboxyalkyl)pyrrole (CAP) epitopes that were detected with enzyme-linked immunosorbent assays using antibodies raised against keyhole limpet hemocyanin (KLH)-bound 2-(ω-carboxyheptyl)pyrrole (CHP) and 2-(ω-carboxypropyl)pyrrole (CPP). These antibodies exhibit high structural selectivity (
