Technical Note pubs.acs.org/ac
Cell Chemotaxis on Paper for Diagnostics David I. Walsh, III,† Mark L. Lalli,‡ Juliette M. Kassas,§ Anand R. Asthagiri,†,‡ and Shashi K. Murthy*,‡,∥ †
Department of Bioengineering, ‡Department of Chemical Engineering, §Department of Biology, ∥Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, Massachusetts, United States ABSTRACT: Microfluidic chemotaxis platforms have historically been utilized to probe phenomena such as neutrophil migration and are beginning to be developed for diagnostic applications; however, current microfluidic chemotaxis systems require specialized engineering equipment such as syringe pumps and long time frames (hours) to develop a chemokine gradient, and cell chemotaxis typically requires multiple additional hours. The paperfluidic device described in this work is a low-cost, sharp (2 mm wide), quasi-stable (at least 20 min) and rapidly generated (90%) in these devices.14 Although these devices provide alternative solutions to cell enumeration for diagnostics, their complexity, high cost, and requirement for dedicated systems such as pumps for operation create a need for new innovative methods that may be performed at the point-of-care for rapid diagnosis. Furthermore, there exist © XXXX American Chemical Society
Received: February 22, 2015 Accepted: May 4, 2015
A
DOI: 10.1021/acs.analchem.5b00726 Anal. Chem. XXXX, XXX, XXX−XXX
Technical Note
Analytical Chemistry
activated cell sorting (e.g., removing monocytes from buffy coat). Other performance gains from using a paper-based format include raw materials cost ($1.18 per paper device vs $9.98 per plastic microfluidic chip), no costly syringe pumps, and fast gradient generation (
