WEBINARS
Purity Analysis of Bispecifc Antibodies by Afnity Capillary Electrophoresis
Stronger bonds.
SEPTEMBER 17, 2014 8:00 a.m. PDT / 11:00 a.m. EDT / 16:00 BST
SPEAKER
MODERATOR
Jiann Kae “Henry” Luo, PhD., Senior Staf Scientist, Protein Biochemistry, Regeneron Pharmaceuticals
Stu Borman, Senior Correspondent, C&EN
OVERVIEW
SPONSOR
Monoclonal antibodies represent an important class of therapeutics for various diseases. Tere is a growing interest in increasing the versatility of monoclonal antibodies, with one approach being the design and generation of bispecifc antibodies (BsAbs). A BsAb possesses two diferent antigen binding sites, which can be useful for targeting strategies or increasing potency. One class of bispecifc antibody has the format of a conventional antibody, with two distinct heavy chains of diferent binding specifcities. Co-expression of these components will typically also result in formation of undesirable homodimeric monospecifc antibodies, which must then be removed in the purifcation process. In order to assess the efciency of the purifcation procedure, a capillary zone electrophoresis (CZE) assay was developed to specifcally identify small amounts of the homodimers in the purifed bispecifc samples. Te CZE assay takes advantage of a high resolution separation method frst described by He and co-workers [1], and incorporates the use of immunoafnity ligands to provide identity to the peaks separated by CE. Tis CZE method ofers high resolution, short experimental times and broad applicability to many diferent BsAbs. [1] He, Y., Lacher, N.A., Hou, W., Wang, Q., Isele, C., Starkey, J. and Ruesch, M. (2010)Anal. Chem. 82: 3222-30.
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SCIEX Separations, Part of AB SCIEX
WHO SHOULD ATTEND Analytical Development Scientists Researchers/ R&D Managers Laboratory Managers/ Directors / Supervisors Laboratory Technicians / Operators
KEY LEARNING OBJECTIVES Te use of CZE in analytical characterization of protein therapeutics Importance of bispecifc antibody analysis Application of Partial Fill Afnity CE for purity analysis
