Article pubs.acs.org/JAFC
Characterization of Soybean Storage and Allergen Proteins Affected by Environmental and Genetic Factors Savithiry Natarajan,*,† Farooq Khan,‡ Qijian Song,† Sukla Lakshman,§ Perry Cregan,† Roy Scott,∥ Emerson Shipe,⊥ and Wesley Garrett# †
Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, Maryland 20705, United States Department of Plant Science & Landscape Architecture, University of Maryland, College Park, Maryland 20742, United States § Diet, Genomics and Immunology Laboratory, USDA-ARS, Beltsville, Maryland 20705, United States ∥ Crop Production and Protection, Oilseeds & Bioscience, USDA-ARS, Beltsville, Maryland 20705, United States ⊥ Clemson University, Department of Entomology, Soils, & Plant Sciences, Clemson, South Carolina 29634, United States # Animal Biosciences and Biotechnology Laboratory, USDA-ARS, Beltsville, Maryland 20705, United States ‡
S Supporting Information *
ABSTRACT: There is limited information on the influence of genetic and environmental variability on soybean protein composition. This study aimed to determine the role of genotype (G), environments (E), and the interrelationship of genotype and environment (G×E) on soybean seed protein. Three sets of nine soybean genotypes were grown in replicated trials at Maryland, South Carolina, and South Dakota. At each location, the nine genotypes were grown with two planting/sowing dates. We applied two-dimensional gel electrophoresis and mass spectrometry to study the variability of soybean storage and allergen proteins. Statistical analysis of 47 storage and 8 allergen proteins, in terms of differentially expressed protein spots significant at the p
