2 μΑ
Substrate
ΓΛΛΛΛ
Water/Air Inlet
ι
Θ
j j · - Enzyme Pad Holder Enzyme Pad Platinum Electrode »- Waste
Figure 3. Electrochemical cell for C A M
Water Supply
...Its the easy, economical way to order H P columns
Buffer Supply
(vV^ Pump
Auto Diluter
Process Digital Pump-Diluter Assembly Dialysis Sampler
Check the quality Instrument: Hewlett-Packard 5831A Column:
Numerical Readout •
53.1
Immobilized Glucose Oxidase
Γ
Detector (H2O2)
6' χ 2 m m ID (glass) 2 % OV-101 on 100/ 120 mesh (Chromosorb W-HP)
Compare the price
Pump
A packed 6' χ 2mm ID glass c o l u m n costs only $54.*
Filter Immobilized Enzymes for Sucrose Enzyme Analyzer
How, order Specify columns, supports, phases, packing, liners, adapters, and test mixes for your HEWLETT-PACKARD GC. If you need a catalog to simplify ordering, just ask. We'll send you one. Fast.
Figure 4. Diagram of Enzymax sucrose/glucose analyzer with accessories
pound butyrlthiocholine iodide to form t h e electroactive thiocholine io dide, which is readily oxidized at t h e platinum probe. If the enzyme be comes inactivated, there will be no electroactive species in solution, and a corresponding change in signal is ob served a t t h e electrochemical cell. Such is t h e case when enzyme inhibi tors such as organophosphates and carbamates are present in t h e sample stream, and this unique approach serves t o illustrate t h e versatility of fered by immobilized enzymes in ana lytical chemistry.
'For U.S. domestic customers only.
BUY BY THE NUMBEBS:
800-523-7133 (IN PA, CALL COLLECT: 215-268-2077)
HEWLETT ^ MÈË
Detector (H2O2)
PACKARD 43704
L e e d s and Northrup C o .
I
T h e family of Enzymax analyzers
CIRCLE 96 ON READER SERVICE CARD 1070 A · ANALYTICAL CHEMISTRY, VOL. 49, NO. 12, OCTOBER 1977
consists of single- and dual-channel glucose analyzers, a dual-channel glu cose/sucrose and a dual-channel glu cose/lactose instrument. T h e analysis of sugars is of particular importance in t h e food processing industry, and the instruments were specifically de signed for continuous on-stream pro cess control. A dual-stream glucose/sucrose ana lyzer shown in Figure 4 illustrates t h e flow scheme common to all the instru ments. A continuous sample is re moved from a process stream through a dialysis thimble. After dilution with a buffer the stream is split and passes through packed beds of immobilized enzyme reagents. T h e enzyme is, of
