Chemical Analysis of Fermentable Sugars and Secondary Products in

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Chemical Analysis of Fermentable Sugars and Secondary Products in 23 Sweet Sorghum Cultivars Minori Uchimiya, Joseph Edward Knoll, William Frank Anderson, and Karen R. Harris-Shultz J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b00675 • Publication Date (Web): 03 Aug 2017 Downloaded from http://pubs.acs.org on August 3, 2017

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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

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Journal of Agricultural and Food Chemistry

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Chemical Analysis of Fermentable Sugars and

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Secondary Products in 23 Sweet Sorghum Cultivars

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Minori Uchimiya*,a, Joseph E. Knollb, William F. Andersonb, and Karen R. Harris-Shultzb

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a

USDA-ARS Southern Regional Research Center, 1100 Robert E. Lee Boulevard, New Orleans, LA 70124

b

USDA-ARS Crop Genetics and Breeding Research Unit, 115 Coastal Way, Tifton, GA 31793

*Corresponding

author

fax:

(504)

286-4367,

phone:

[email protected] (M. Uchimiya)

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(504)

286-4356,

email:

Journal of Agricultural and Food Chemistry

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Abstract

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Sorghum (Sorghum bicolor (L.) Moench) is a heat- and drought-tolerant crop that has promise to

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supplement corn (Zea mays L.) for biofuel production from fermentable sugars (for sweet cultivars) and

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lignocellulosic biomass. Quantitative relationships are lacking to predict the accumulation of primary

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(stem sugars) and secondary (organic acids, phenolics, and inorganic species) products that could either

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expand (as the value-added product) or limit (as the fermentation inhibitor) the market value of a

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cultivar. Five male (Atlas, Chinese, Dale, Isidomba, N98) and three female (N109B, N110B, and

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N111B) inbred lines and their hybrids (23 cultivars total) were planted on a Tifton loamy sand in April,

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May, and June of 2015 in a triplicate split-plot design, and were harvested at the hard-dough maturity

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stage. Stalk juices were analyzed for sugar (glucose, fructose, and sucrose) and organic acid (citrate,

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oxalate, and cis- and trans-aconitic acid) concentrations, Brix, pH, electric conductivity (EC), total

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organic carbon (TOC) and total nitrogen (TN), and by fluorescence excitation emission

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spectrophotometry with parallel factor analysis (EEM/PARAFAC).

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(p