ATÀLE OF FOUR CHEMISTS Λ\Β/ ot too long ago, four chemists were γ working in four fabs. ^M I All four encountered analysis problems: the first chemists problem concerned metals, the seconds had to do with organic ions, the thirds involved amino acids, ana the fourths was with common inorganic anions. While our chemists pondered their analysis problems, a common solution was already waiting for them...
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N-Methyl Pyrrolidine (NMP) in a n Antibiotic
T h e Metals Story
NMP
Conductivity Detection
"I had to analyze a variety of transition metah at extremely low concentration leveL·. Interferences were always a problem"
Trace Metals in Electroless Plating Bath
"My sample load and research needs are such that AA and ICP alone aren't sufficient. We needed to increase the efficiency of our electroless nickel plating solutions for the manufacture of magnetic storage media. Of particular importance were transition metals: lead, cadmium, cobalt, copper, and nickel. However, monitoring hypophosphite and organic acid concentrations was crucial too. To my delight, I discovered that a Dionex system can analyze all of these and speciate metals and other ions according to their oxidation states. "Our Dionex virtually eliminated the old interference problems and gave us the sensitivity we needed to improve our production yields. Now we even do our routine wastewater samples on our Dionex."
U V Detection (254nm)
T h e Organic Story
"The ionic organics I was analyzing had no chromophores and were difficult to chromatograph. Conventional HPLCjust couldn't do the job" Comparison of Detection Modes
in the Analysis of Milk Dionex Conductivity
UV(2l5nm) (Sample (Concentrated l()\) Unknown
1. Citrate
2. Pyruvate
Sample Name :Electroless Nickel Bath #32 PEAK
RET
PEAK
CONC.
NUH TIME
WWE
ug/1
1 2 3
2.13 2.95
LEW
Ml
CADMIUM
«
6.77 12.53
5 Minutes
COPPER COBALT NICKEL
a Lactate
in
1.216E+Î2 9.34βΕ««1 9.55«Ε+β1 1.271Ε*β3 9.844Ε*β6 Minutes
Minuits
Minutes
"We do a lot of work for clients in the food and pharmaceutical industries. Naturally, we depend on LC and GC. But there are cases where these tech niques won't separate and detect the compo nents we're analyzing. "For example, many organic amines are diffi cult to chromatograph. We've found that a Dionex Ion Chromatograph is an easy solution to this tough problem. "In this case, our Dionex enabled us to determine an amine impurity in an antibiotic, something we couldn't do with silica-based columns and UV detection. "Highly complex samples, like milk, have always been a problem. O u r Dionex is able to detect organics in milk with better selectivity and sensitivity."
The Inorganic Story
My Dionex Ion Chromatograph beats them all. "With our high sample load, we simply couldn't cope without unattended sample preparation, analysis, and data reduction. We get all of those from o u r Dionex systems, with consistent accuracyit doesn't matter whether we're analyzing sub-partper-billion concentrations in ultra-pure water or percentage-level concentrations in turbid solutions. O u r chromatograms speak for themselves." Anions in Water
The Amino Acid Story
Hydrolyzed Feed Sample
Minutes
"I need to determine amino acids, carbohydrates, and anions in my samples. I got tired of running from instrument to instrument!' "To get all three profiles, we had to r u n samples through a series of procedures on several different instruments. O u r expensive dedicated amino acid analyzer could only do part of the job.
"Now I can do it a i l automatically if I w a n t on a single instrument: a Dionex Ion Chromatograph. " O u r Dionex gives us excellent resolution of amino acids in record time, and we can switch to carbohydrates or anions at the touch of a button'.' Carbohydrates in Food
Minutes
"In our lab we have to analyze a variety of common anions in a large number of samples, and it always has to be done yesterday" Minutes
"My specialty is water analysis, and I've tried every method: wet chemistry, automatic titrators, ion-selective electrodes. I've even modified an H PLC with an ion exchange column and a conductivity detector.
DIONEX THE COMMON SOLUTION Dionex systems are solving tough problems in all areas of analytical chemistry. Turn your analysis problems into success stories:
Metals Analysis Dionex lets you determine transition metals, metal complexes, and mono- and divalent cations at trace- and high percent-level concentrations, directly from acid or alkaline digests or brines, even in the presence of other major metal constituents. You can also speciate metal oxidation states such as Fe'-,+/Fe:,+ and Cr 3 + /Cr 6 + . And with Dionex automatic precolu m n concentration, you can analyze as many as nine metals in a single run at concentrations below parts-per-billion.
allow trace quantitation of amines, organic acids, sugar alcohols, carbohydrates, and chelates that have little UV absorbance. Dionex systems can solve problems that HPLC and GC can't. Of course, you can use standard HPLC] columns on a Dionex for your routine HPLC work as well.
derivatization and your choice of anion or cation exchange modes. In addition, you can use your Dionex system to analyze inorganic ions, transition metals, and organic ions as well as amino a c i d s all in a single instrument.
Amino Acid Analysis
Dionex Ion Chromatography has made tedious wet chemical analysis methods obsolete. Furthermore, single-column techniques simply can't match the performance and versatility of a Dionex. T h a n k s to advanced membrane-based detection technologies, Dionex guarantees sub-ppb sensitivity and the highest specificity for all common anions. Dionex technology
New high-speed pellicular ion exchange resins combined with metal-free instrumentation make Dionex Ion Chromatography more versatile, faster, and more reliable than other amino acid analysis systems. You get highly specific ion exchange separation with automatic post-column
Inorganic Analysis
also gives you high precision analysis for assaying complex solutions such as plating baths, Kraft liquors, foods, and paints.
Automation Improve your lab's productivity by adding the convenience and consistent accuracy of Dionex automation. With multim o d e switching, you can go from organics to metals to anions at the touch of a button. Add the Autolon 300 computer for multipleinstrument control and you can further increase your lab's productivity and sample throughput. Dionex brings true automation to chromatography—in sample preparation, unattended analysis, and data handling. For the complete story or for a demonstration, contact your nearest Dionex representative or call us direct.
Organic Analysis H igh-efficiency Dionex ion exchange resins are revolutionizing organic ion separations. O u r unique ion detection systems
Dionex Corporation PO. Box 3603 Sunnyvale, CA 94088-3603 (408) 737-0700
Itasca, IL (312) 773-6850 Atlanta, GA (404) 953-8981
DIONEX THE O N EXPERTS
Houston,TX (713) 847-5652 Marital), NI (609) 596-0600
Dionex (UK) Ltd. Farnborough, Hants England (0252)541346
©Dionex Corporation 1985
Dionex GmbH West Germany 06150-3047
Semsa, S.A. Boulogne, France (1)621 66 66
Dionex S.r.l. Roma, Italy (06) 536130
LPN 32555 3/85
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