Coherent Brightfield Microscopy Provides the Spatiotemporal Resolution To Study Early Stage Viral Infection in Live Cells Yi-Fan Huang,† Guan-Yu Zhuo,† Chun-Yu Chou,† Cheng-Hao Lin,† Wen Chang,‡ and Chia-Lung Hsieh*,† †
Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 10617, Taiwan Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan
‡
S Supporting Information *
ABSTRACT: Viral infection starts with a virus particle landing on a cell surface followed by penetration of the plasma membrane. Due to the difficulty of measuring the rapid motion of small-sized virus particles on the membrane, little is known about how a virus particle reaches an endocytic site after landing at a random location. Here, we use coherent brightfield (COBRI) microscopy to investigate early stage viral infection with ultrahigh spatiotemporal resolution. By detecting intrinsic scattered light via imaging-based interferometry, COBRI microscopy allows us to track the motion of a single vaccinia virus particle with nanometer spatial precision (
