Biochemistry 1988, 27, 5565-5573
5565
Comparative Studies of the Sugar Chains of Aminopeptidase N and Dipeptidylpeptidase IV Purified from Rat Kidney Brush-Border Membrane? Katsuko Yamashita,t Yoko Tachibana,l Yoshiko Matsuda," Nobuhiko Katunuma," Naohisa Kochibe,I and Akira Kobata* Department of Biochemistry, Kobe University School of Medicine, Chuo-ku, Kobe, Hyogo 650, Japan Received December 17, 1987; Revised Manuscript Received February 26, 1988
ABSTRACT: Asparagine-linked sugar chains of rat kidney aminopeptidase N and dipeptidylpeptidase
IV
were investigated comparatively. Oligosaccharides released from the two enzymes by hydrazinolysis were fractionated by paper electrophoresis, serial chromatographies on columns of immobilized Aleuria aurantia lectin, concanavalin A, phytohemagglutinin Ed,and Datura stramonium agglutinin, and Bio-Gel P-4 (
