Conformation-dependent carbon-13 NMR chemical shifts of poly(L

Benjamin J. Wylie, W. Trent Franks, and Chad M. Rienstra ... Jeffrey M. Njus, Ramaswamy Nagarajan, Ashok L. Cholli, Lynne A. Samuelson, and Jayant Kum...
0 downloads 0 Views 646KB Size
457

Macromolecules 1984,17, 457-461

Conformation-Dependent I3C NMR Chemical Shifts of Poly(L-alanine) in the Solid State: FPT INDO Calculation of N-Acetyl-N'-methyl-L-alanineAmide as a Model Compound of Poly(L-alanine) Isao Ando,*+Hazime Sait6,' Ryoko Tabeta,t Akira Shoji,l and Takuo Ozakig Department of Polymer Chemistry, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo, Japan 152, Biophysics Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan 104, and Department of Industrial Chemistry, College of Technology, Gunma University, Tenjin-cho, Kiryu-shi, Gunma, Japan 376. Received February 14, 1983

ABSTRACT An attempt is made to calculate 13CNMR chemical s h of N-acetyl-N'-methyl-L-alanine ~ amide, a model compound for poly(L-alanine),as functions of the dihedral angles 4 and $ specifying the peptide chain, by using the FPT INDO method, in order to obtain a clue as to the origin of the conformation-dependent 13CNMR chemical shift previously determined for solid oligob-alanines)and poly(L-alanine),and copolymers of L- and D-alanines by the cross-polarization/magicangle spinning technique. It is found that the calculated results on C,, C,, and carbonyl carbons exhibit conformation-dependent13Cchemical shifts comparable with the experimental data.

Introduction Recent 13C NMR studies of solid p~lypeptides,l-~ as measured by the cross-polarization/magic angle spinning (CP/MAS) t e ~ h n i q u e ? ~demonstrated that 13C NMR chemical shifts of the C,, C,, and carbonyl carbons are considerably displaced, up to 7 ppm, depending on the particular conformations adopted, such as a-helix and 0-sheet forms. The existence of such conformation-dependent 13Cchemical shifts is very valuable in examining whether or not the conformation of the polypeptide under consideration is retained in the solution state. This approach can be extended to probe local conformations of amino acids in peptides6,' and proteinsspg by taking into account the finding that 13Cchemical shifts of an amino acid residue in random coil conformation are effectively independent of the neighbors except for the presence of proline residues.'O It is reasonable to ascribe conformation-dependent 13C chemical shifts to changes of electronic structure accompanying changes of the dihedral angles of the skeletal peptide bonds. To prove this view, we attempted to calculate relative 13C chemical shifts of a dipeptide fragment of poly(L-alanine)," N-acetyl-N'-methyl-L-alanineamide, employing the finite perturbation INDO (FPT INDO) theory.15J6 It is sufficient to employ such a dipeptide fragment in the elucidation of the chemical shift behavior of poly(L-alanine) and oligo(L-alanines), and copolymers of L- and D-alanines because the chemical shift is sensitive in most cases to the local structure. Such an attempt, therefore, is valuable in understanding the origin of 13C chemical shifts as well as in providing insight into 13C chemical shifts of unstable conformers which are experimentally unattainable.12J7

from the remaining atoms. The dominant factor usually governing 13Cchemical shifts is u whereas UdAA makes only a small contribution and @ may be negligible because it is unlikely to exceed a few parts per million. The FPT INDO theory has the advantage of permitting the calculation of the paramagnetic term without the use of the explicit wave function for excited states, which cannot be obtained accurately by an ordinary SCF MO theory. For this reason, the FPT INDO theory has successfully explained the 13C chemical shifts of various organic compounds.18 According to the FPT INDO theory,l5?l6adAA and upM are expressed by

upAA =

a,P = x , Y , z (3)

where the gauge origin of the vector potential is set at the position of nucleus A. The vectors rv and rAare the position vectors of an electron considered from a nucleus of the atom containing the atomic orbital xvand from the nucleus A, respectively. PJH,) and PJO) are the elements of the density matrix with and without the perturbation due to the magnetic field, H,respectively. These are obtained by solving the Roothaan equation with the HartreeFock matrix, FPv(Ha),containing the perturbation terms in the INDO framework.

Theoretical Section The magnetic shielding, &, of nucleus A in a molecule with atoms A, B, and others may be written a d 6 ffA

=

udAA

+ upAA + B ( # N

(TAB

(1)

where adAA and upAAare the local diamagnetic and paramagnetic terms, respectively, and OAB is the contribution 'Tokyo Institute of Technology. National Cancer Center Research Inatitute. 5 Gunma University.

*

0024-9297/84/2217-0457$01.50/0

In this equation, the first two terms on the right-hand side are the ordinary Hartree-Fock matrix and the last term is the perturbation due to the magnetic field, which is given (pvlXu), and ( ~ J ~ X Yare ) as an imaginary number. WF, the unperturbed core Hamiltonian, Coulomb integral, and exchange integral, respectively. These are estimated by parameters within the INDO framework. In the present work, we shall adopt Pople's parameter in the INDO 0 1984 American Chemical Society

458 Ando e t al.

Macromolecules, Vol. 17, No. 3, 1984

The chemical shift, Pod,for the random-coil conformation is related to the mole fraction p Land the characteristic chemical shift, a,, of individual preferred conformers by bco'l = Cp$, (7) 1

The calculation for P1 was carried out by the averaging of bcol' with p L ,which is computed from the total energy of conformer i obtained from the INDO calculation. HITAC M 200H computers at the Computer Center of the Tokyo Institute of Technology and at the Computer Center of the Institute for Molecular Science were used for the calculation. Results and Discussion 13CChemical Shifts of (Ala),. Figure 2 shows the 13C chemical shift contour map of the C,, C,, and carbonyl carbons of N-acetyl-N'-methyl-L-alanine amide for model , I, calculated by the FPT INDO theory, as a function of the dihedral angles 4 and $ whose values were varied in 15' intervals. The chemical shift is expressed in ppm, which was interpolated from the calculation at 15' intervals. The right-handed and left-handed a-helix (CURand cyL, respectively) regions, and the antiparallel and parallel 0-sheet (PA and Pp, respectively) regions are indicated by solid circle, as deduced from X-ray studies made on poly(L-alanine).21,22 H We first consider the contour map for the C, carbon (Figure 2a), because the chemical shift of the C, carbon Figure 1. The molecular structure of N-acetyl-N'-methyl-Lalanine amide: (a) model I; (b)model I1 (taking hydrogen bonds is not strongly affected by the formation of a hydrogen with two formamide molecules), 4 = $ = 180'. bond, as described below. The chemical shift in the a R helix form appears at higher field than that in the aL-helix method except for the off-diagonal element of the core form, and the variation of the chemical shift with the Hamiltonian, which is estimated from the expression dihedral angles 4 and IC, in the vicinity of the former is larger than that of the latter. On the other hand, both the (5) B y = (Kr/2)(P0A + P'B)Spp positions for the PA- and &sheet forms fall on the same contour, so their chemical shifts are nearly equal. Thus, in which K , denotes a correlation factor for the T-T inthe chemical shifts for PA- and pp-sheet forms appear at teraction, PoAthe bonding parameter for atom A, and s,, lower field than those in the a-helix forms and @A- and the overlap integral. In the present calculation, a param&-sheet forms cannot be distinguished from each other eter set which reproduces fairly well the chemical shift by the chemical shifts for the C, carbon. The numerical of hydrocarbons was used: PoH = -13 eV, Poc = -15 eV, values of the 13Cchemical shifts of aR-and aL-helixforms, @ON = -25 eV, and Poo = -31 eV for hydrogen, carbon, and PA- and op-sheet forms are summarized in Table I. nitrogen, and oxygen atoms, respectively.16 The differential coefficient in eq 3 is replaced numeriNext, we consider the contour maps for the C, and carbonyl carbons (Figure 2b,c). It appears, as far as the cally by C, carbon is concerned, that the chemical shift for the a R form is at lower field than that for the aL form (the difference between them being about 1ppm) and there is also a significant displacement between the P A and pp forms, in constrast to the case of the C, carbon. Accordingly, the where Im (PJH,)) denotes the imaginary part of the PA- and pp-sheet structures could be distinguished through density matrix PJHJ. These molecular integrals were the chemical shift of the C, carbon. On the other hand, evaluated by means of the Gaussian-transform method the contour map for the carbonyl carbon shows that the presented by Karplus et al.19 chemical shift for the aR-helixform appears at lower field We adopted the molecular geometry of N-acetyl-N'than that for the aLform and is at higher field than those methyl-L-alanine amide from the values proposed by for PA- and pp-sheet forms. Further, the chemical shift for Momany et aL20 For the calculation, the dihedral angles the PA-sheetform appears at lower field than that for the @ and IC. (Figure 1) are varied at 15' intervals. It appears pp-sheet form. Although some features of the contour obvious that this calculation (model I) may not be good maps for the C, and carbonyl carbons have been described, enough for the C, and carbonyl carbons, because the inthere appears to be, as already suggested above, a limitatramolecular and intermolecular hydrogen bonds for the tion in the application of the maps to solid poly(L-alanine) a-helix and @-sheetforms, respectively, might strongly because no hydrogen bonding is taken into account. For affect the chemical shifts of these carbons. Thus, we the sake of comparison, we summarize pertinent numerical performed an alternative calculation on a model system values in Table I. In addition, the chemical shifts for the (model 11) in which two formamides are hydrogen bonded random coil, occurring in solution, were also estimated on to the amide groups of the dipeptide in an appropriate geometry with the a-helix and @-sheetforms (Figure 1). the basis of the treatment of eq 7 by using the above contour maps and the total energy (Table I). The structural data, including the distance between niAs an alternative model which takes into account hytrogen and oxygen atoms, 2.83 and 2.87 8, for the p-sheet drogen bonding in the a-helix and p-sheet, we performed and a-helix forms, respectively, were taken from X-ray diffraction studies of poly(L-alanines) by Arnott et a1.21~22 numerical calculation of chemical shifts on the basis of

13C NMR

Macromolecules, Vol. 17,No. 3, 1984

Chemical Shift of Poly(L-alanine) 459

(4

D

E01 D

3

c

@',degree

(b)

9119

I)i

I\

I

180

120

60 0

?0 -

0

3 4v.P

-60

" 3

W -1 2

LJ -18 -180

'

'

'

-120

,/'

'

-60

'

I

'

0

-89 i -430 +bo

'

'

'

60

'I '1, 120

180

@ .degree

-1Bo 0

-180

I l L " -4&&+ MAb*! JJ -hL

-120

-60

0

60

120

180

@ ,degree

Figure 2. The calculated chemical shift contour maps for the C, (a), C, (b), and carbonyl carbons (c) in N-acety1-N'methyl-Lalanine amide. The methyl end groups were held fixed as shown in Figure 1. The chemical shifta were calculated at 15' intervals in 4 and $. model 11(Table I). It is found that hydrogen bonding leads to an upfield shift for the C, and carbonyl carbon but does not effectively change the chemical shift of the C, carbon. To assist comparison with the experimental data, we reproduce some '3c NMR spectra taken in the solid state.3 It is seen from Figure 3A,B and Table I that the calculated chemical shifts of the C, carbon on the basis of model I are paralleled by the experimental data in the case of the aR-helix,BA-sheet,and random coil (in trifluoroacetic acid solution) forms in poly(L-alanine)%while those of the C,

75

50

25

ppmo

Figure 3. lsC CP/MAS NMR (75.46 MHz) spectra of random copolymers of L-alanine and D-danine, and poly(L-alanines)in solid state: (A) (Ala),; (B) (Ala), (n = 2800), (C) copolymer LID = 91/9;@) copolymer LID = 59/41; (E) copolymer LID = 50/50, (F) copolymer LID = 22/78. These spectra were reorganized with use of the method published in ref 3. For the experimental conditions see ref 3. and carbonyl carbons are not. (It is known from X-ray diffraction study that the @-sheetform of poly(L-alanine) has antiparallel.21*22) In particular, the difference in chemical shift of the C, carbon between the aR-helixand OA-sheetforms is 2.6 ppm, in good agreement with the experimentalfinding of 5.0 ppm. In addition, the chemical shift of the random coil lies between those of the aR-helix and PA-sheetforms. Therefore, it is clear that the chemical shifts of the C, carbon can be mainly explained in terms of the conformational change. However, the calculated chemical shifts of C, and carbonyl carbons on the basis of model I conflict with the experimental data. I t appears that the cause of such disagreement is mainly our choice of model I, neglecting the hydrogen bonding at the amide group, which plays an important role in stabilizing these forms. As shown in Table I, the results of model I1 show that the formation of the hydrogen bonds at the amide groups causes an upfield displacement for both the carbonyl and C, carbons. Such an effect is more significant for the BA-sheetform than for the aR-heliX form (Table I), because in these hydrogen bonds the distance between the nitrogen and oxygen atoms is reported to be shorter for the latter.21v22 Thus, the difference in the calculated chemical shifts between the aR-helixand BA-sheetforms is 1.6 and 2.1 ppm for the C, and carbonyl carbons, respectively, and is in agreement with the experimental values (4.2 and 4.6 ppm, respecitvely). Inclusion of the hydrogen bond does not affect the nuclear shielding on the C, carbon (slightly downfield shift by 0.1 ppm). Application of the Contour Map to the Elucidation of the Conformational Behavoir of Random Co-

460 Ando et al.

Macromolecules, Vol. 17, No. 3, 1984

Table I I3C Chemical Shifts of Alanine Peptides Characteristic of a-Helix, @Sheet,and Random Coil Forms (in ppm) calcd obsda (poly(L-alanine)) a -helix 0-sheet random carbon a-helix P-sheet coil‘ model CYR aL PA BP random coil 14.9 19.9 157 I -63.0 -63.7 -65.6 -65.3 -64.3 c!3 I1 -63.1 -63.6 -64.7 -65.4 -63.2d c, 52.4 48.2 51.1 I -88.5 -88.1 -87.7 -89.2 -88.2 I1 -87.8 -86.4 -86.2 -85.5 -86.gd CO 176.4 171.8 176.1 I -184.2 -182.2 -185.7 -185.1 -185.2 11 -183.1 -181.7 -182.3d -181.0 -185.6 ~~~~~

a From tetramethylsilane. Reference 3. Shielding constant f o r N-acetyl-N’-methyl-L-alanineamide. solution. Averaged over CUR, a L , pA, and ~p forms.

polymers of L- and D-Alanines. In our previous work3, we have reported that when the proportion of L-alanine (or D-alanine) is increased in random copolymers of L- and D-alanine, additional peaks (marked by i) appear besides the C=O and C, signals of the aR-helix form (marked by a). It appears, however, that such a change is not seen in the C, signal region. It is plausible that the additional peak in this case is accidentally superimposed on the a-helical C, signal. The anomaly of the distribution of the peak intensities previously mentioned3is easily resolved by this new assignment. In parallel with this finding by the CP/MAS data, we found that two peaks of far-infrared spectra (spectra not shown), 420 and 478 cm-’, are increased at the expense of the a-helix peaks (374 and 526 cm-’) when the proportion of L- or D-ahine residues in the copolymers is increased. In addition, the peak intensity of the characteristc band of the PA-sheet(442 cm-’) was less than 5 1 0 % of the new peaks mentioned above. This observation is in good agreement with that by Itoh et al.23 They established the assignment of the infrared peaks (420 and 478 cm-’) to D-alanine residues incorporated into the right-handed a-helix or L-alanine residues in the lefthanded a-helix residues. On the basis of the infrared data, it is now clear to assign the peaks i of the CP/MAS spectra to the L- or D-alanine residues incorporated into the leftor right-handed a-helices, respectively, in spite of the similarity of the peak positions to the P-sheet form (C=O and C, region).24 It is worthwhile to examine whether such an assignment is justified or not on the basis of the contour map as a test of the usefulness of this approach. The aR-helixform in poly(L-alanine) and the aL-helixform in poly(D-alanine) lie at the same position (aR)in the contour maps where 4 = -48’ and $ = -57°.21922,25 On the other hand, the D-alanine residues incorporated into the aR-helixin a Lalanine sequence or L-alanine residues into the aL-helix in a Dalanine sequence lie on the same position (aL) in the contour maps where 4 = 48’ and $ = 57°.23The chemical shifts of the C, carbon in the aL-helix appear at the same field as in the aR-helix,whereas those of the C, and carbonyl carbons in the aL-helixappear at higher field than in the aR-helix. The chemical shifts of the C, and carbonyl carbons in the aL-helixshift in the direction of the @*-sheet form (Figure 2 and Table I). These trends are more pronounced when the hydrogen bond is taken into account. The peaks i of the C=O and C, region thus should be at positions very close to those of the @-sheetform, in good agreement with the experimental finding. Downfield displacement of the C, signal in the D or L-alanine residues incorporated into the aR-helix is rather small compared with those of the C, and C=O region (Table I), resulting in the overlap with the original C, signal. This trend is again consistent with our experimental finding. We find that the calculation of the contour map is very useful as

~~~

In CF,COOD

a means of demonstrating the presence of the D-alanine residues incorporated into the aR-helix in an L-alanine sequence or of L-alanine residues incorporated into the aL-helixin a D-alanine sequence (4 = 48’ and $ = 57’), as shown from the 13C chemical shift values. In conclusion, we have shown that conformation-dependent 13C chemical shifts of poly(i-alanines) may be mainly interpreted in terms of the change of the electronic structure brought about by changes in the dihedral angles of the skeletal bonds and also by the intra- or interchain hydrogen bonds. Registry No.N-Acetyl-N’-methyl-L-alanineamide, 19701-83-8; poly(L-alanine) (homopolymer), 25191-17-7;poly(L-alanine) (SRU), 25213-34-7.

References and Notes T. Taki, S. Yamashita, M. Satoh, A. Shibata, T. Yamashita, R. Tabeta, and H. SaitB, Chem. Lett., 1903 (1981). D. Muller and H. R. Kricheldorf, Polym. Bull., 6, 101 (1981). H. SaitB, R. Tabeta, A. Shoji, T. Ozaki, and I. Ando, Mucromolecules, 16, 1050 (1983). J. Schaefer and E. 0. Stejskal in “Topics in Carbon-13 NMR Spectroscopy”, G. C. Levy, Ed., Vol. 3, Wiley-Interscience, New York, 1979, p 283. J. R. Lyerla in ‘Contemporary Topics in Polymer Science”, M. Shen, Ed., Vol. 3, Plenum Press, New York, 1979, p 143. L. G. Pease, M. H. Frey, and S. J. Opella, J. Am. Chem. SOC., 103, 467 (1981). L. M. Gierasch. M. H. Frev. J. G . Hexem.‘ and S. J. ODella. ACS Symp. Ser., No. 191, i 3 3 (1982). H. Saitb, M. Kamesama, M. Kodama. and C. Napata. J. Biochem. (Tokyo), 92,-233 (1982). H. Sait6, Y. Iwanaga, R. Tabeta, M. Narita, and T. Asakura, Chem. Lett., 427 (1983). 0. W. Howarth and D. M. J. Lilley, Prog. Nucl. Magn. Reson. Spectrosc., 12, l(1978). Previously, Tonelli12 explained the variation of 13C chemical shifts of various peptides in solution in terms of the gauche y-effect arising from the interaction with the substituent in the y p~sition.’~~’~ For the interpretation of chemical shifts by this concept, the dipeptide fragment used here may not be large enough to describe the conformation-dependent 13C chemical shifts of the carbonyl carbons. However, our view is that 13C chemical shifts are determined by the electronic structure of the conformer under consideration, which is a function of the dihedral angle (6 and $) as well as hydrogen bonds between NH and C=O groups. At present, it is not clear whether the gauche y-effect arises from the conformational effect or a nonbonded through-space effect or both. If the former is dominant, it is possible to obtain the conformation-dependent I3C chemical shifts even in the dipeptide fragment, including the term corresponding to the gauche yeffect. Quantitatively, similar results were obtained in our separate calculation based on the tight-binding MO approximation in which infinite chain length of polypeptide is taken into account (T. Yamanobe, I. Ando, H. SaitB, R. Tabeta, A. Shoji, and T. Ozaki, Mol. Phys., submitted for publication). A. E. Tonelli and F. C. Schilling, Acc. Chem. Res. 14, 233 (1981). A. E. Tonelli, J. Am. Chem. SOC., 102, 7635 (1980). F. A. Bovey in “Proceedings of the International Symposium on Macromolecules, Rio de Janeiro, July 26-31, 1974, E. B. I

I

Macromolecules 1984, 17, 461-465

461

dried quickly, are very close to those of the 0-sheet form, although the C, signal is identical with that of the right-handed a-helical (Asp(OBzl),J. (H. Saito, R. Tabeta, I. Ando, T. Ozaki, and A. Shoji, Chem. Lett., 1437 (1983).) This observation strongly supports our present assignment of the peaks i. As to the assignment of the peak i in the C, carbon, one of the reviewers raised a question as follows. When a D-alanine adopts the aRhelix its Ct carbon and carbonyl oxygen atoms are eclipsed, or cis. This overlapped arrangement of C, is removed to L-alanine in the aR-helix. It is pointed out in ref 7 that this eclipsed arrangement is highly shielding and would be expected to result in the D-alanine C, resonance appearing upfield from the C, resonance in L-alanine (aR-helix). This view, however, is incorrect as viewed from the present experimental finding as well as that of (Asp(0Bzl)J. It is also emphasized that the variation of 13C chemical shifts, similar to that of (Ala),, is well reproduced by a theoretical calculation (I. Ando et al., manuscript in preparation). (25) F. A. Bovey in “Macromolecules,An Introduction to Polymer Science”, F. A. Bovey and F. H. Winslow, Eds., Academic Press, New York, 1979, Chapter 8.

Mano, Ed., Elsevier, Amsterdam, 1975, p 169. R. Ditchfield, D. P. Miller, and J. A. Pople, J. Chem. Phys., 54, 4186 (1971). M. Kondo, I. Ando, R. ChGj6, and A. Nishioka, J. Magn. Reson., 24, 315 (1976). I. Ando and T. Asakura, Annu. Rept. N M R Spectrosc., 10A, 81 (1980). K. A. K. Ebraheem and G. A. Webb, Bog. Nucl. Magn. Reson. Spectrosc. 11, 149 (1977). (a) I. Shavitt and M. Karplus, J. Chem. Phys., 43,398 (1965); (b) C. W. Kern and M. Karplus, ibid.,43, 415 (1965). F. A. Momany, R. F. McGuire, J. F. Yan, and H. A. Scheraga, J. Phys. Chem., 75, 2286 (1971). S. Arnott and A. L. Wonacott, J. Mol. Biol., 21, 371 (1966). S. Arnott, S. D. Dover, and A. Elliot, J. Mol. Biol., 30, 201 (1967). K. Itoh, T. Nakahara, T. Shimanouchi, M. Oya, K. Uno, and Y. Iwakura, Biopolymers, 6, 1759 (1968). Recently, we have observed that the C, and carbonyl 13C chemical shifts of the left-handed a-helical poly(@-benzyl+ aspartate) (Asp(OBzl),), cast from chloroform solution and

Isotactic Polymerization of C-3Branched a-Olefins: Conformation of the Monomer1 A. Zambelli,* P. Ammendola, and A. J. Sivak2 Zstituto Chimico dell’Universit6 di Napoli, 80134 Naples, Italy. Received May 31, 1983

ABSTRACT: The rate constants of the initiation on metal-methyl bonds for isotactic polymerization of 3-methyl-1-pentene (3MP1), 3-methyl-1-butene (3MB1), and 3-ethyl-1-pentene (3EP1) have been compared to each other. Their relative values are accounted for by assuming that the monomers react with a possibly distorted H-skew or H-skew’ conformation.

1. Introduction In a previous paper it was pointed out that insertion of 3-methyl-1-pentene (3MP1) into the reactive metal-carbon bond of the active sites of Ziegler-Natta isotactic-specific catalysts is diastereoseledive. It was actually observed that the R’,R and S‘,S faces of the racemic monomer are twice as reactive as the S’,R and R’,S faces (Figure 1)both in the enantioselective chain propagation steps and in the nonenantioselective initiation on Mt-CH, If one considers the most favored solution-state conformation of the olefin (H-syn)- (see Figure 2), it turns out that the more reactive faces of 3MP1 (R‘,R and S’,S) also appear to be the most hindered. On the other hand, it is quite possible that the monomer is forced to achieve a different conformation in order to approach the reactive metal-carbon bond of the active site with a minimum of nonbonded interactions. In this paper the reactivity of the diastereotopic faces of 3MP1 in the initiation on Mt-CH, bonds is compared with the reactivity of the enantiotopic faces of 3-methyl1-butene (3MB1) and 3-ethyl-1-pentene (3EP1). Some information concerning the conformation of the monomer in the active state is inferred. 2. Experimental Section Reagents. 3MB1 was prepared according to the l i t e r a t ~ r e . ~ (RS)-3MP1 and 3EP1 were commercial products. The purity of the monomers was checked by GLC (>98%). The monomen and the polymerization diluent (pentane) were distilled under vacuum in the presence of a small amount of Al(n-C,H,), just before polymerization. 13C-enriched(34%) Al(CH,), and Zn(CH& were prepared according to ref 10. 6-TiC1, was prepared according to the literature’l (elemental analyak Ti, 20.18; Al, 7.45; C1,69.60). 0024-9297/84/2217-0461$01.50/0

Copolymerization of 3-1 and (RS)-3MPl. Five milliliters of anhydrous pentane, 4.9 X lo-, mol of &Tic&, 1.2 X lo-, mol of A1(l3CHJ3, and 2.9 X lo-, mol of Zn(13CH3)2were introduced under nitrogen into a 60-mL reactor provided with a magnetic stirring bar and cooled to -78 OC. The reactor was then cooled with liquid nitrogen and evacuated with a diffusion pump. Next, mol of 3MB1 were 1.2 X lo-’ mol of (RS)-3MP1 and 2.6 X condensed into the reactor through a vacuum line. The reactor was transferred into a thermostated bath. After 5 h of stirring a t 50 “C the polymerization was stopped by injecting 5 mL of 2-ethylhexanol into the reactor. Polymer yield was 0.65 g. Copolymerization of 3 E P 1 and (RS)-3MPl. The copolymerization was performed as above, with 5 mL of pentane, mol of A1(l3CH3),, 3.1 X lo-, 6 X lo-, mol of 6-TiC13, 1.3 X mol of (RS)-3MPl, and 9.2 X mol of Zn(13CH3)2,6.2 X mol of 3EP1. Polymerization time was 20 h; yield was 0.4 g. Fractionation. The copolymers were fractionated by subsequent exhaustive extractions with boiling diethyl ether and toluene.12 The results are reported in Table I. ’% NMR Analysis. Proton-noise-decoupled ’% NMR analysis of the samples dissolved in 1,2,4trichlorobenzene containing 20% tetrachloroethane-l,2-dzfor field-frequency stabilization and HMDS as an internal standard was carried out at 130 “C in the PFT mode on a Varian XL-200 spectrometer operating a t 50.309 MHz. Pulse width was 4.7 ps. The fractions insoluble in boiling toluene were thermally degraded as described in ref 3. X-ray Analysis. X-ray spectra of unoriented samples were obtained with a Philips APD spectrometer using Cu Kru radiation. Both the toluene-soluble fractions and the toluene-insoluble fractions were found to have nearly the same degree of crystal1inity .

3. Results (RS)-3MP1has been copolymerized with 3MB1 or 3EP1 in the presence of the isotactic-specific catalytic system

0 1984 American Chemical Society