Addition/Correction pubs.acs.org/JPCB
Correction to “Deoxygenation Increases Photoluminescence Lifetime of Protein-Responsive Organic Probes with Triplet−Singlet Resonant Energy Transfer” Kadri Ligi, Erki Enkvist, and Asko Uri* J. Phys. Chem. B 2016, 120 (22), 4945−4954. DOI: 10.1021/acs.jpcb.6b03342
T
of energy transfer from 1D* to 1A is very high, approximately 70−90%. The authors would like to apologize to the readers of the article for any confusion caused by these mistakes.
he original version of the article unfortunately contained two mistakes. (1) A calculation mistake was made in the course of the normalization of values of I0*τ for ARC-Lum/PKAc complexes (Table 1, p 4948 in the original paper). Also, the value of I0 for Se(Cy3B) was re-evaluated. Here we would like to present the corrected Table 1. (2) The explanation following eq 3 (p 4951 in the original paper) was misleading. Here we are presenting the corrected text with the equation. Equation 3 was used for the calculation of the efficiency of energy transfer:1 E=
⎤ εA (λDex ) ⎡ FAD(λAem) − 1⎥ ex ⎢ em εD(λD ) ⎣ FA(λA ) ⎦
■
REFERENCES
(1) Lakowicz, J. R. Energy Transfer Efficiency From Enhanced Acceptor Fluorescence. In Principles of Fluorescence Spectroscopy; Springer: Boston, MA, 2006; pp 461−462.
(3)
Here, E is the efficiency of the energy transfer; εA(λex D ) and εD(λex D ) are the molar extinction coefficients of the acceptor and donor at the excitation wavelength of the donor, respectively. FAD(λAem) and FA(λAem) are the fluorescence emission intensities of the acceptor in the presence and absence of the donor at the emission wavelength of the acceptor, respectively. The normalized excitation spectrum (rather than the absorption spectrum) was applied for the determination of εA(λex D ). The estimated efficiency Table 1. Luminescence Decay Times (τ, μs), Normalized Luminescence Intensity (I0), and Normalized Integrated Luminescence Intensity (I0*τ) of ARC-Lum Probes in PK-Bound Statea Se(−) components added to the probe + PKAc
Se(Cy3B)
Se(PF647)
S(PF647)
τ (μs)
I0
I0*τ
τ (μs)
I0
I0*τ
τ (μs)
I0
I0*τ
τ (μs)
I0
I0*τ
111 ± 7
1.0 ± 0.1
1.0 ± 0.1
77 ± 1
28 ± 5
19 ± 3
36 ± 3
240 ± 50
77 ± 18
101 ± 4
6.9 ± 0.5
6.3 ± 0.7
108 ± 5
1.0 ± 0.1
1.0 ± 0.1
76 ± 2
37 ± 8
26 ± 6
33 ± 1
280 ± 20
83 ± 8
109 ± 5
6.6 ± 0.4
6.4 ± 0.8
447 ± 5
1.2 ± 0.1
4.7 ± 0.6
157 ± 4
39 ± 4
55 ± 7
47 ± 1
270 ± 30
112 ± 16
556 ± 5
7.5 ± 1.0
37 ± 6
+G + PKAc + GO + C + PKAc + GO + C + G a
The data are based on measurements performed with the plate reader PHERAstar at 30 °C in the presence of dissolved molecular oxygen and in enzymatically deoxygenated conditions. The transmittance of the optical filters as well as the concentration of the probe applied for the measurements have been considered for normalization of I0 of ARC-Lum/PKAc complexes. The normalization of I0 and integrated luminescence intensity (I0*τ) have been done in relation to Se(−)/PKAc complex in the presence of dissolved molecular oxygen. Error bars represent SEM (N = 2 to N = 5). Published: September 23, 2016 © 2016 American Chemical Society
10421
DOI: 10.1021/acs.jpcb.6b09086 J. Phys. Chem. B 2016, 120, 10421−10421
