Addition/Correction pubs.acs.org/biochemistry
Correction to Hysteresis and Negative Cooperativity in Human UDP-Glucose Dehydrogenase Renuka Kadirvelraj, Nicholas C. Sennett, Gregory S. Custer, Robert S. Phillips, and Zachary A. Wood* Biochemistry 2013, 52 (8), 1456−1465. DOI: 10.1021/bi301593c Several corrections are needed in Table 2: in Row 3, column 2, “(0.12 ± 3) × 10−3 (k4)b” should be changed to “0.120 ± 0.003 (k4)b”, in Row 3, column 3, “(2 ± 1) × 10−3 (k−4)b” should be changed to “0.002 ± 0.001 (k−4)b”, in Row 4, column 3, “105 × 10−4 ± 1.5 × 10−3 (k−5)a” should be changed to “0.0105 ± 0.0015 (k−5)a”, and “E†:UDG:NADH ↔ E*:UDG:NADH” should be changed to “E:UDG:NADH ↔ E*:UDG:NADH”. The corrected Table 2 with these changes incorporated appears below. Table 2. Microscopic Rate Constants from hUGDH Transient-State Kinetics kforward (kf, s−1)
transient event †
†
E :UDG + NADH ↔ E :UDG:NADH E†:UDG:NADH ↔ E*:UDG:NADH E + NADH ↔ E:NADH E:UDG:NADH ↔ E*:UDG:NADH
−1
kreverse (kr, s−1)
a
2.7 ± 0.1 M (k3) 0.120 ± 0.003 (k4)b 2.7 × 10−4 ± 1.5 × 10−5 M−1 (k5)a 42.7 ± 1.4 (k7)b
a
b
a
21.9 ± 2 (k−3) 0.002 ± 0.001 (k−4)b 0.0105 ± 0.0015 (k−5)a 0.26 ± 1.3 (k−7)b
kr/kf 8.1 0.017 39 0.006
Keq 79.3 (K3)b 58.3 (K6)b
Forward and reverse rate constants were calculated using eq 8. Forward and reverse rate constants and the equilibrium constant (Keq) were calculated using eq 9.
Published: January 8, 2015 © 2015 American Chemical Society
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DOI: 10.1021/bi501527p Biochemistry 2015, 54, 629−629
