Correction to Multiple Src Homology 3 Binding to the Ubiquitin Ligase

Addition/Correction pubs.acs.org/biochemistry

Correction to Multiple Src Homology 3 Binding to the Ubiquitin Ligase Itch Conserved Proline-Rich Region Guillaume Desrochers, Mathieu Lussier-Price, James G. Omichinski, and Annie Angers* Biochemistry 2015, 54 (50), 7345−7354. DOI: 10.1021/acs.biochem.5b01131

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uring the course of revising this work, the panels of Figure 5 were rearranged; as a result, the mask in the

Figure 5. Itch PRR cannot accommodate two SH3 domains simultaneously. The His-Itch PRR bound to Ni-NTA agarose was added to an equimolar concentration of soluble GST-Endophilin SH3 (top) or GST-β-PIX (bottom). Bound GST fusions were competed by further addition and incubation with the indicated untagged SH3 domain at 0, 10, 100, or 1000 times the concentration of the GSTtagged SH3 domains. The resin was extensively washed, and the amount of bound GST-SH3 domains was determined by Western blotting with anti-GST antibodies.

bottom panel was not shifted. Both panels ended up showing the same portion of the original blot and thus do not correspond to their description in the text. This change does not alter any conclusion, as they were based on the correct figure. At the same time, we wish to modify the figure legend of Figure 3, so that the figure is easier to interpret. Figure 3. Direct binding of the Itch PRR to SH3 domaincontaining proteins. (A) Far Western blot analysis of protein extracts from HEK-293T cells transfected with the indicated GFP fusion with SH3 domain-containing proteins or nontransfected (NT) cells as a control. Proteins were separated by SDS−PAGE in triplicate and transferred on nitrocellulose membranes. The first set was analyzed with anti-GFP antibodies to determine protein expression levels (blot panel). The second and third sets were incubated with the GST-Itch PRR (middle panels) or GST alone (right panels) before the levels of bound fusion protein were determined with anti-GST antibodies. (B) Far Western blot analysis of protein extracts from HEK-293T cells transfected with untagged Amphiphysin or nontransfected (NT) cells as a control. Extracts were processed as described for panel A, except that the first panel was blotted with anti-Amphiphysin antibodies.

© 2015 American Chemical Society

Received: May 20, 2016

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DOI: 10.1021/acs.biochem.6b00515 Biochemistry XXXX, XXX, XXX−XXX