Addition/Correction pubs.acs.org/NanoLett
Correction to Stimuli-Triggered Activity of Nanoreactors by Biomimetic Engineering Polymer Membranes Tomaz Einfalt, R. Goers, I. A. Dinu, A. Najer, M. Spulber, O. Onaca-Fischer, and C. G. Palivan* Nano Lett. 2015, 15 (11), 7596−7603. DOI: 10.1021/acs.nanolett.5b03386 S Supporting Information *
In addition, the Supporting Information file has been updated to reflect changes in the legend of the Figure S5.
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n the original paper, the legends in Figures 2 and 5 were incorrect. The revised legends are shown below. Figure 2. Characterization of OmpF modification. (a) Carbonyl content determination of OmpF-CA, OmpF-WT, OmpF-CA+Cy5, and OmpF-WT+Cy5 protein pores, as determined by a DNPH protein carbonylation kit, and (b) molecular brightness (CPM) of OG micelles with various OmpFs and/or free dye in 3% OG or PBS: (1) OmpF-CA+Cy5, 3% OG pH 7.2 (dark blue), (2) OmpF-CA+Cy5, 3% OG pH 5.5 (blue), (3) OmpF-WT+Cy5, 3% OG pH 7.2 (dark red), (4) OmpF-WT+Cy5, 3% OG pH 5.5 (red), (5) Cy5, 3% OG pH 7.2 (dark gray), (6) Cy5, 3% OG pH 5.5 (gray), and (7) Cy5 pH 7.4 (black). Figure 5. TMB conversion kinetics measured at pH = 7.4 of nanoreactors prepared with different OmpF subsets: (1) unpermeabelized nanoreactors incubated at pH = 5.5 (orange), (2) unpermeabelized nanoreactors incubated at pH 7.4 (green), (3) OmpF-CA-Cy5 nanoreactors incubated at pH 7.4 (blue), (4) OmpF-CA-Cy5 nanoreactors incubated at pH 5.5 (gray), (5) OmpF-CA nanoreactors incubated at pH 5.5 (red), (6) OmpF-WT nanoreactors incubated at pH 5.5 (black), (7) OmpF-WT nanoreactors incubated at pH 7.4 (dark blue). In the original paper, the formula of the levulonic acid in Scheme 2 was incorrect. The revised figure is shown here.
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ASSOCIATED CONTENT
* Supporting Information S
The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.nanolett.5b04911. Detailed experimental methods regarding the expression, purification and modification of OmpF, preparation and characterization of the nanoreactors, FCS data and enzyme kinetics data (PDF)
Scheme 2. Chemical Modification of Wild Type OmpF with a pH Sensitive Molecular Cap (By Coupling with Levulinic Acid and Cy5-Hydrazide)
Published: December 8, 2015 © 2015 American Chemical Society
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DOI: 10.1021/acs.nanolett.5b04911 Nano Lett. 2016, 16, 823−823
