Addition/Correction pubs.acs.org/biochemistry
Correction to The Nuclease Domain of the Escherichia Coli RecBCD Enzyme Catalyzes Degradation of Linear and Circular SingleStranded and Double-Stranded DNA Jian-Zhong Sun, Douglas A. Julin, and Jin-Shan Hu* Biochemistry 2006, 45 (1), 131−140. DOI: 10.1021/bi051150v Figure 2 in the original publication is actually a duplicate of Figure 4. The correct Figure 2 appears below.
Figure 2. Nuclease reactions of wild-type and mutant RecB30 proteins with a circular ssDNA substrate. The reactions were conducted at 25 °C by incubating 0.4 μg of M13mp18 ssDNA (25 nM) with 20 nM wild-type RecB30 or mutant RecB30, or 20 units of E. coli exonuclease I in the nuclease reaction buffer as described in Materials and Methods, and the samples were analyzed on a 1% agarose gel. Lane 1 shows the reaction with E. coli ExoI nuclease for 60 min. Lanes 2−9 show the reactions with wild-type RecB30 for 0, 0.5, 1, 2, 5, 10, 30, and 60 min, respectively. Lanes 10 and 11 show the reactions with RecB30D1067A (Mut1) and RecB30D1080A (Mut2) mutant proteins for 60 min, respectively. Lane 12 (M) contained the GeneRuler 100 bp DNA Ladder Plus marker (Fermentas, Inc.).
Received: August 31, 2016
© 2006 American Chemical Society
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DOI: 10.1021/acs.biochem.6b00861 Biochemistry XXXX, XXX, XXX−XXX
