Addition/Correction pubs.acs.org/ac
Correction to Ubiquitin Chain Enrichment Middle-Down Mass Spectrometry Enables Characterization of Branched Ubiquitin Chains in Cellulo Sean O. Crowe, Ambar S. J. B. Rana, Kirandeep K. Deol, Ying Ge, and Eric R. Strieter* Anal. Chem. 2017, 89, 4428−4434. DOI: 10.1021/acs.analchem.6b03675 We would like to correct the order in which the figures appear in our original paper. During the editing process, the images for Figure 2 and Figure 3 were inadvertently switched such that the
Figure 3. Middle-down MS analysis of Ub chains isolated from HEK cells using Halo-NZF1 resin. (A) ESI MS spectra showing the MS spectra of chains isolated using Halo-NZF1 resin. The spectra show the presence of all three minimally digested products: Ub1−74 (green box), GGUb1−74 (red box), and 2xGGUb1−74 (blue box). The spectra correspond to the Ub11+ charge state for chains isolated from either untreated or MG132 treated HEK lysate. (B) Quantification of Ub1−74 species after different lengths of proteasome inhibition. The percent distribution is calculated by averaging relative abundance of each Ub1−74 species to total abundance of all three species from three biological replicates for each treatment (Table S3). Error bars represent the SEM for each data set. Calc’d, calculated most abundant weight; Expt’l, experimental most abundant molecular weight.
Figure 2. Middle-down MS analysis of Ub chains isolated from HEK cells using agarose bound TUBE2 shows dynamics in Ub chain branching upon proteasome inhibition. (A) ESI TOF MS spectra showing the presence of all three minimally digested products: Ub1−74 (green box), GGUb1−74 (red box), and 2xGGUb1−74 (blue box). The spectra correspond to the Ub11+ charge state for chains isolated from either untreated or MG132 treated HEK lysate. (B) Quantification of Ub 1−74 species at different MG132 time points. The percent distribution is calculated by averaging relative abundance of each Ub1−74 species to total abundance of all three species from three biological replicates for each treatment (Table S2). Error bars represent standard error of the mean (SEM) for each data set: *, p < 0.05; **, p < 0.01; ***, p < 0.001 (Student’s t test). Calc’d, calculated most abundant weight; Expt’l, experimental most abundant molecular weight.
figures do not correspond to their respective captions. We would like to correct this error so that the figures and captions agree. The correct order should be as follows.
© XXXX American Chemical Society
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DOI: 10.1021/acs.analchem.7b02855 Anal. Chem. XXXX, XXX, XXX−XXX
