Published on Web 06/23/2007
Covalent Immobilization of Peptides on Self-Assembled Monolayer Surfaces Using Soft-Landing of Mass-Selected Ions Peng Wang, Omar Hadjar, and Julia Laskin* Fundamental Science Directorate, Pacific Northwest National Laboratory, Richland, Washington 99352 Received March 14, 2007; E-mail:
[email protected] Covalent immobilization of peptides on solid supports plays an important role in biochemistry with applications ranging from characterization of molecular recognition events at the amino acid level and identification of biologically active motifs in proteins to development of novel biosensors and substrates for improved cell adhesion.1 Self-assembled monolayers (SAMs) provide a simple and convenient platform for tailoring chemical properties of surfaces.2 Existing techniques for linking peptides to SAMs are based on solution-phase synthetic strategies and require relatively large quantities of purified material.3 Here, we report a novel approach for highly selective covalent binding of peptides to SAMs using soft-landing (SL) of mass-selected ions. SL is defined as intact deposition of ions onto suitable substrates at hyperthermal (
