Chapter 8
Cranberry Extract as a Bacterial Anti-adhesive Coating 1
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Terri A . Camesano , Jeffrey R. Pouliot , , Joel B. Lofgren1,4, and Paola A . Pinzon-Arango 2
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Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, M A Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, M A Current Address: Amgen, Inc., West Greenwich, RI Current Address: Boston University School of Law, Boston, M A 2
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Cranberry extract in solutions of 0, 20, 60, and 100% was applied as a coating to silicone rubber, which simulated the material of a urinary catheter. The attachment of Escherichia coli JR1 to the treated polymers was quantified under flow conditions, using artificial urine as the suspending phase. Increasing the concentration of cranberry extract that was applied to the polymer decreased the attachment of E. coli. The polymer was sampled in 15 or 30 minute intervals from 0 to 180 minutes. N o trend with time could be seen in the number of bacteria attaching for any of the cranberry extract concentrations, over the range of 15 to 180 min, since it appeared that the number of attached bacteria reached a steady-state within the first 15 min. The coatings with varying cranberry extract concentrations produced statistically different numbers of bacteria attaching, according to the Kruskal-Wallis one-way analysis of variance on the ranks (P
