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Delphinidin prevents muscle atrophy and upregulates miR-23a expression Motoki Murata, Haruna Nonaka, Satomi Komatsu, Megumi Goto, Mai Morozumi, Shuhei Yamada, I-Chian Lin, Shuya Yamashita, and Hirofumi Tachibana J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.6b03661 • Publication Date (Web): 21 Dec 2016 Downloaded from http://pubs.acs.org on December 24, 2016
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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Delphinidin Prevents Muscle Atrophy and Upregulates miR-23a Expression
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Motoki Murata, Haruna Nonaka, Satomi Komatsu, Megumi Goto, Mai Morozumi,
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Shuhei Yamada, I-Chian Lin, Shuya Yamashita and Hirofumi Tachibana*
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Division
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Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
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*Corresponding author. 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
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Running title: Delphinidin prevents disuse muscle atrophy by induction of miRNA
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Journal of Agricultural and Food Chemistry
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■ABSTRACT
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Delphinidin, one of the major anthocyanidins, shows protective effects against a
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variety of pathologies, including cancer, inflammation, and muscle atrophy. The
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purpose of this study was to determine the preventive mechanism of delphinidin on
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disuse muscle atrophy. In vitro and in vivo models were used to validate the effects of
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delphinidin on the expression of MuRF1, miR-23a and NFATc3. Delphinidin suppressed
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the upregulation of MuRF1 (1.77±0.05 vs 1.03±0.17, P < 0.05) expression and
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inhibited the downregulation of miR-23a (0.56±0.05 vs 0.94±0.06, P < 0.05) and
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NFATc3 (0.61±0.02 vs 1.02±0.08, P < 0.01) expression in dexamethasone-treated
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C2C12 cells. In gastrocnemius, muscle weight loss was prevented by oral
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administration of delphinidin. Moreover, delphinidin suppressed MuRF1 (3.35±0.13 vs
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2.26±0.3, P < 0.01) expression and promoted miR-23a (0.58±0.15 vs 2.25±0.29, P
