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Detection and Quantification of Early-Stage Malaria Parasites in Laboratory Infected Erythrocytes by Attenuated Total Reflectance Infrared Spectroscopy and Multivariate Analysis Aazam Khoshmanesh,† Matthew W. A. Dixon,‡ Shannon Kenny,‡ Leann Tilley,‡ Don McNaughton,† and Bayden R. Wood*,† †
Centre for Biospectroscopy and School of Chemistry, Monash University, Clayton, Victoria 3800, Australia Department of Biochemistry and Molecular Biology and Bio21 Molecular, Science and Biotechnology Institute, The University of Melbourne, Melbourne, Victoria 3010 Australia
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S Supporting Information *
ABSTRACT: New diagnostic modalities for malaria must have high sensitivity and be affordable to the developing world. We report on a method to rapidly detect and quantify different stages of malaria parasites, including ring and gametocyte forms, using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR) and partial least-squares regression (PLS). The absolute detection limit was found to be 0.00001% parasitemia (
