J. Agric. Food Chem. 1995, 43, 2580-2584
2580
Determination of 2-(2’-Octenyl)succinicAcid in Lipophilic Modified Starch by Gas Chromatography-Mass Spectrometry/Selected Ion Monitoring Peter W. Park* and Randy E. Goins Mead Johnson Nutritional Group, Mead Johnson Research Center, 2400 West Lloyd Expressway, Evansville, Indiana 47721
The covalent bonding of 2-(2’-octeny1)succinic acid (OSA) to starch enables the resulting modified starch to be used as an emulsifier. A gas chromatography (GC)-mass spectrometry (MS) procedure for free and total OSA quantitation in lipophilic modified starch is described. For free OSA, starch was removed from aqueous dispersion by precipitation with methanol and the acidified supernatant (pHi2) was extracted. For total OSA, esterified OSA was hydrolyzed in an alkaline solution and OSA was extracted following acidification (pH
