Article pubs.acs.org/ac
In-ESI Source Hydrogen/Deuterium Exchange of Carbohydrate Ions Yury Kostyukevich,†,§ Alexey Kononikhin,†,§ Igor Popov,‡,§ and Eugene Nikolaev*,†,‡,∇ †
Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskij pr. 38 k.2, 119334 Moscow, Russia Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Kosygina st. 4, 119334 Moscow, Russia § Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russia ∇ Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, 119121 Moscow, Russia ‡
S Supporting Information *
ABSTRACT: We present the investigation of hydrogen/deuterium (H/D) exchange of carbohydrates ions occurring in the electrospray ion source. The shape of the deuterium distribution was observed to be considerably dependent on the temperature of the ion transfer tube and the solvent used. If deuterated alcohol (EtOD or MeOD) or D2O/deuterated alcohol is used as an electrospray solvent, then for high temperatures (>350 °C), intensive back exchange is observed, resulting in ∼30% depth of the deuterium exchange. At low temperatures (350 °C), the intensive back exchange is observed resulting in ∼30% depth of the deuterium exchange (see Figures 1A and 1D). Under low temperature (350 °C), intensive back exchange is observed, resulting in ∼30% depth of the deuterium exchange. Under low temperatures (
