Development and Application of a Sensitive and Rapid Immunoassay

Development and Application of a Sensitive and Rapid Immunoassay for the Quantitation of N7-Methyldeoxyguanosine in DNA Samples. Kathryn L. Harrison* ...
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Chem. Res. Toxicol. 2001, 14, 295-301

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Development and Application of a Sensitive and Rapid Immunoassay for the Quantitation of N7-Methyldeoxyguanosine in DNA Samples Kathryn L. Harrison,*,†,‡ Michelle Wood,‡ Nicholas P. Lees,§ C. Nick Hall,§ Geoffery P. Margison,‡ and Andrew C. Povey† School of Epidemiology and Health Sciences, Medical School, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom, CRC Carcinogenesis Group, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester M20 4BX, United Kingdom, and Department of Surgery, Wythenshawe Hospital, Wythenshawe, Manchester, United Kingdom Received March 31, 2000

N7-Methyldeoxyguanosine (N7-MedG) in DNA is a biomarker of exposure to environmental and endogenous methylating agents and may be of use in epidemiological studies. To quantitate N7-MedG in human samples, a sensitive assay system that uses only small quantities of DNA (