16 D e v e l o p m e n t of I m m u n o a s s a y s f o r P a r a l y t i c S h e l l f i s h Poisoning
Downloaded by UNIV OF CALIFORNIA SAN DIEGO on December 24, 2015 | http://pubs.acs.org Publication Date: September 19, 1984 | doi: 10.1021/bk-1984-0262.ch016
A Radioimmunoassay for Saxitoxin ROBERT E. CARLSON, MARIE L. LEVER, BRIAN W. LEE, and PATRICK E. GUIRE Bio-Metric Systems, Inc., Eden Prairie, MN 55344 A simple and fast radioimmunoassay for saxitoxin has been developed using an anti-saxitoxinol antibody. The immune serum required for the immunoassay was raised in rabbits using an immunogen conjugate prepared from saxitoxinol and bovine serum albumin with a phosgene based coupling reagent. The antibody, has moderate a f f i n i t y for saxitoxinol (K ~ 5x10 L/mole). It cross-reacts at >90% with saxitoxin but at
