Distance Mapping in Proteins Using Fluorescence Spectroscopy

Aug 21, 2014 - Here we report new advances in this site-directed fluorescence labeling (SDFL) approach, gleaned from recent studies of T4 lysozyme (T4...
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Article pubs.acs.org/biochemistry

Distance Mapping in Proteins Using Fluorescence Spectroscopy: Tyrosine, like Tryptophan, Quenches Bimane Fluorescence in a Distance-Dependent Manner Amber M. Jones Brunette and David L. Farrens* Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97239-3098, United States S Supporting Information *

ABSTRACT: Tryptophan-induced quenching of fluorophores (TrIQ) uses intramolecular fluorescence quenching to assess distances in proteins too small (